Paul.xu at 17:13, 23 December 2020

2020-12-23T17:13:45Z

New page

Use this link to calculate Tris Buffer

[http://www.cytographica.com/lab/HHTris.html http://www.cytographica.com/lab/HHTris.html]

or

'''Trizma Buffer Table – pH vs. Temperature'''

{| cellspacing="1" cellpadding="2" border="1"
|-
| colspan="3" | '''pH at Temperature'''
| rowspan="1" colspan="2" | gL for 1 M Tris-HCl <br/>
| width="200" colspan="3" | '''g/L for 0.05 M Solution'''
|-
| '''5 °C'''
| '''25 °C'''
| '''37 °C'''
| '''Trizma HCl'''<br/>
|  '''Trizma Base'''
| '''Trizma HCL'''
| '''Trizma Base'''
|-
| 7.76
| 7.20
| 6.91
| <br/>
|  
| 7.02
| 0.67
|-
| 7.89
| 7.30
| 7.02
| <br/>
|  
| 6.85
| 0.80
|-
| 7.97
| 7.40
| 7.12
| <br/>
|  
| 6.61
| 0.97
|-
| 8.07
| 7.50
| 7.22
| 127 g
|  23.6 g
| 6.35
| 1.18
|-
| 8.18
| 7.60
| 7.30
| <br/>
|  
| 6.06
| 1.39
|-
| 8.26
| 7.70
| 7.40
| <br/>
|  
| 5.72
| 1.66
|-
| 8.37
| 7.80
| 7.52
| <br/>
|  
| 5.32
| 1.97
|-
| 8.48
| 7.90
| 7.62
| <br/>
|  
| 4.88
| 2.30
|-
| 8.58
| 8.00
| 7.71
| <br/>
|  
| 4.44
| 2.65
|-
| 8.68
| 8.10
| 7.80
| <br/>
|  
| 4.02
| 2.97
|-
| 8.78
| 8.20
| 7.91
| <br/>
|  
| 3.54
| 3.34
|-
| 8.88
| 8.30
| 8.01
| <br/>
|  
| 3.07
| 3.70
|-
| 8.98
| 8.40
| 8.10
| <br/>
|  
| 2.64
| 4.03
|-
| 9.09
| 8.50
| 8.22
| <br/>
|  
| 2.21
| 4.36
|-
| 9.18
| 8.60
| 8.31
| <br/>
|  
| 1.83
| 4.65
|-
| 9.28
| 8.70
| 8.42
| <br/>
|  
| 1.50
| 4.90
|-
| 9.36
| 8.80
| 8.51
| <br/>
|  
| 1.23
| 5.13
|-
| 9.47
| 8.90
| 8.62
| <br/>
|  
| 0.96
| 5.32
|-
| 9.56
| 9.00
| 8.70
| <br/>
|  
| 0.76
| 5.47
|}

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| 0.2 M TrisMa(24.2 g/L)
| 0.2 M HCl
| pH after dilution to 200 ml
|-
| 50 ml
| 5.0 ml,  or 86 ul of 12 N HCl
| 9.0
|-
| 50 ml<br/>
| 8.1 ml, or  139  ul of 12 N HCl
| 8.8
|-
| 50 ml<br/>
| 12.2 ml, or  209  ul of 12 N HCl
| 8.6
|-
| 50 ml<br/>
| 16.5 ml, or  283 ul of 12 N HCl
| 8.4
|-
| 50 ml<br/>
| 21.9 ml, or  376  ul of 12 N HCl
| 8.2
|-
| 50 ml<br/>
| 26.8 ml, or  460 ul of 12 N HCl
| 8.0
|-
| 50 ml<br/>
| 32.5 ml, or  558 ul of 12 N HCl
| 7.8
|-
| 50 ml<br/>
| 38.4 ml, or  659 ul of 12 N HCl
| 7.6
|-
| 50 ml<br/>
| 41.4 ml, or   711 ul of 12 N HCl
| 7.4
|-
| 50 ml<br/>
| 44.2 ml, or   759 ul of 12 N HCl
| 7.2
|}

<div><br/></div>

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| 0.1 M Tris Buffer pH 
| Tris-HCl,   (mw: 157.6 g/mol) <br/>
| Tris Base, Trizma Base (mw: 121.14 g/mol)
|-
| 7.2
| <div>14.77<br/></div>
| 0.77<br/>
|-
| 7.3
| <div>14.0<br/></div>
| 1.36         <br/>
|-
| 7.4
| <div>13.23</div>
| 1.95<br/>
|-
| 7.5
| <div>12.47<br/></div>
| 2.53<br/>
|-
| 7.6
| <div>11.7<br/></div>
| 3.12<br/>
|-
| 7.7
| 10.94<br/>
| 3.71<br/>
|-
| 7.8
| 10.17<br/>
| 4.29<br/>
|-
| 7.9
| 9.41<br/>
| 4.88<br/>
|-
| 8.0
| 8.65<br/>
| 5.47<br/>
|-
| 8.1
| 7.88<br/>
| 6.05<br/>
|-
| 8.2
| 7.12<br/>
| 6.64<br/>
|-
| 8.3
| 6.36<br/>
| 7.23<br/>
|-
| 8.4
| 5.59<br/>
| 7.82<br/>
|-
| 8.5
| 4.83<br/>
| 8.40<br/>
|-
| 8.6
| 4.07<br/>
| 8.99<br/>
|-
| 8.7
| 3.30<br/>
| 9.58<br/>
|-
| 8.8
| 2.54
| 10.16<br/>
|-
| 8.9
| 1.78
| 10.75<br/>
|-
| 9.0
| 1.01<br/>
| 11.34<br/>
|}

[https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0 https://www.aatbio.com/resources/buffer-preparations-and-recipes/trizma-buffer-ph-7-2-to-9-0]

1 M TRIS pH 7.5 (1 L):  TRIS HCl 127 g  + TRIS Base 23.6 g

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| 1 M TrisMa, Dissolve 121.14 g of Tris (Tris Base) in 750 ml H2O, adjust pH to at 25 oC
| Concentrated HCl, ml
|-
| 7.0
| 77<br/>
|-
| 7.1
| 75                <br/>
|-
| 7.2
| 73<br/>
|-
| 7.3
| 72<br/>
|-
| 7.4
| 70<br/>
|-
| 7.5
| 67<br/>
|-
| 7.6
| 64<br/>
|-
| 7.7
| 61<br/>
|-
| 7.8
| 57<br/>
|-
| 7.9
| 53<br/>
|-
| 8.0
| 49<br/>
|-
| 8.1
| 45<br/>
|-
| 8.2
| 41<br/>
|-
| 8.3
| 37<br/>
|-
| 8.4
| 32<br/>
|-
| 8.5
| 28<br/>
|-
| 8.6
| 25<br/>
|-
| 8.7
| 21<br/>
|-
| 8.8
| 18<br/>
|-
| 8.9
| 15<br/>
|-
| 9.0
| 12<br/>
|}

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| 0.1 M Tris-HCl (12.1g trisma in 1 L final vol), Final pH at 25°C
| 0.1 N HCl
|-
| 7.2
| 89.4mL<br/>
|-
| 7.3
| 86.8<br/>
|-
| 7.4
| 84.0<br/>
|-
| 7.5
| 80.6<br/>
|-
| 7.6
| 77.0<br/>
|-
| 7.7
| 73.2<br/>
|-
| 7.8
| 69.0m<br/>
|-
| 7.9
| 64.0<br/>
|-
| 8.0
| 58.4<br/>
|-
| 8.1
| 52.4<br/>
|-
| 8.2
| 45.8<br/>
|-
| 8.3
| 39.8<br/>
|-
| 8.4
| 34.4<br/>
|-
| 8.5
| 29.4<br/>
|-
| 8.6
| 24.8<br/>
|-
| 8.7
| 20.6<br/>
|-
| 8.8
| 17.0<br/>
|-
| 8.9
| 14.0<br/>
|}
<div>12.472.53</div><div>11.73.12</div><div>10.943.71</div><div>10.174.29</div><div>9.414.88</div><div>8.655.47</div><div>7.886.05</div><div>7.126.64</div><div>6.367.23</div><div>5.597.82</div><div>4.838.40</div><div>4.078.99</div><div>3.309.58</div><div>1.7810.75</div><div>1.0111.34</div>[[Category:Buffer Recipe]]

Tris Buffer - Revision history

Paul.xu at 17:13, 23 December 2020