https://eln.excellgen.com/index.php?action=history&feed=atom&title=Trouble_report Trouble report - Revision history 2026-10-19T08:31:11Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Trouble_report&diff=18913&oldid=prev Paul.xu at 13:43, 2 August 2018 2018-08-02T13:43:19Z <p></p> <p><b>New page</b></p><div>{| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width: 693px;&quot;<br /> |-<br /> | #<br /> | Name<br /> | Antibiotics<br /> | Vector<br /> | Enzymes<br /> | Status<br /> | style=&quot;width: 1px;&quot; | Date<br /> | style=&quot;width: 7px;&quot; | possible reasons for failure<br /> | style=&quot;width: 241px;&quot; | Solution or&amp;nbsp;remedy<br /> |-<br /> | Subclone<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | style=&quot;width: 1px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 7px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 241px;&quot; | &lt;br/&gt;<br /> |-<br /> | 931<br /> | pT7A3-PAK16D ligase&lt;br/&gt;<br /> | K<br /> | 931-1(927 as vector)<br /> | Nde1+HindIII<br /> | doing<br /> | style=&quot;width: 1px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 7px;&quot; | the vector has two bands after 1 hour digest, might contaminated?<br /> | style=&quot;width: 241px;&quot; | digest with fastdigest 1 hour&lt;br/&gt;<br /> |-<br /> | PCR cloning<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | style=&quot;width: 1px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 7px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 241px;&quot; | &lt;br/&gt;<br /> |-<br /> | 3206<br /> | pEF1-HTLV-LAMP-1&lt;br/&gt;<br /> | Amp<br /> | 3165<br /> | BamH1+Not1<br /> | miniprep digestion no insert<br /> | style=&quot;width: 1px;&quot; | 7/31/18&lt;br/&gt;<br /> | style=&quot;width: 7px;&quot; | no insert<br /> | style=&quot;width: 241px;&quot; | &lt;br/&gt;<br /> |-<br /> | 998<br /> | pT7A3-6His-NLS-dCas9-NLS-6xHis&lt;br/&gt;<br /> | K<br /> | 1708<br /> | HindIII+Xho1<br /> | &lt;br/&gt;<br /> | style=&quot;width: 1px;&quot; | 7/31/18&lt;br/&gt;<br /> | style=&quot;width: 7px;&quot; | &lt;br/&gt;<br /> | style=&quot;width: 241px;&quot; | &lt;br/&gt;<br /> |-<br /> | 3235<br /> | pCMV5-IL2-MICA-(3c)-Fc-WPRE-OriP&lt;br/&gt;<br /> | Amp<br /> | 3237 or 3232<br /> | Nhe1+Kpn1<br /> | <br /> | style=&quot;width: 1px;&quot; | <br /> | style=&quot;width: 7px;&quot; | just start to do&amp;nbsp;<br /> | style=&quot;width: 241px;&quot; | &lt;br/&gt;<br /> |}</div> Paul.xu