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TurboTEV - Revision history
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Luyingying at 13:14, 28 October 2011
2011-10-28T13:14:12Z
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<p><b>New page</b></p><div>Cloning of Turo-TEV <br />
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Purpose: to clone Turo-TEV <br />
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Date: 2011-10-14 <br />
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Notebook:Liang Huo<br> <br />
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Page: <br />
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Clone ID:203 <br />
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Experimenters: Liang Huo <br />
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<br>Results:<br> <br />
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20 oligo first round Two PCR reactions, then Using outside primers second round PCR, fragement ~800bp <br />
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[[Image:IMG 1593 1.JPG|115x238px|IMG 1593 1.JPG]] <br />
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EcorI+Sal1 digest pET28b and PCR&nbsp;fragement, gel purified, ligation O.N at 16 degree.<br> <br />
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8 clones screening( after ECoRI and Sal1 digested): <br />
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[[Image:TurboTEV-203.JPG]] <br />
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Picked clone 3 for sequencing, using T7 term as seq primer<br />
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[[Category:Cloning]]</div>
Luyingying