Paul.xu: Created page with "Vaccinia DNA polymerase can be purified by. A working stock of polymerase was prepared by diluting it to a concentration of 25 ng/mL in polymerase dilution buffer.
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2012-07-27T16:41:14Z

Created page with "Vaccinia DNA polymerase can be purified by. A working stock of polymerase was prepared by diluting it to a concentration of 25 ng/mL in polymerase dilution buffer. <br>A ..."

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Vaccinia DNA polymerase can be purified by.  A working stock of polymerase was prepared by diluting it to a concentration of 25 ng/mL in polymerase dilution buffer.

<br>A C-terminal His6-tagged form of recombinant vaccinia single-strand DNA binding protein (I3)  E. coli strain BL21 and purified using nickel affinity columns as described by Tseng et al. (18). The purity was confirmed by SDS-PAGE, and the concentration determined using a Bradford assay. To produce a working stock, the I3 DNA binding protein was diluted to a concentration of 0.5 mg/mL in PBS.<br><br>

[[Category:Protocols]]

VVDP - Revision history

Paul.xu: Created page with "Vaccinia DNA polymerase can be purified by. A working stock of polymerase was prepared by diluting it to a concentration of 25 ng/mL in polymerase dilution buffer. A ..."

Paul.xu: Created page with "Vaccinia DNA polymerase can be purified by. A working stock of polymerase was prepared by diluting it to a concentration of 25 ng/mL in polymerase dilution buffer.
A ..."