https://eln.excellgen.com/index.php?action=history&feed=atom&title=Vector_vs_Sequencing_Primers
Vector vs Sequencing Primers - Revision history
2026-10-18T07:56:01Z
Revision history for this page on the wiki
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Crm at 14:42, 8 June 2018
2018-06-08T14:42:54Z
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<p><b>New page</b></p><div>{| border="1" cellspacing="1" cellpadding="1" style="width:800px;"<br />
|-<br />
| Vector Name<br />
| Clone ID<br />
| style="width: 178px;" | Description<br />
| style="width: 223px;" | 5' Primer<br />
| Primer Lot, Location<br />
| 3' Primer<br />
| Primer Lot, Location<br />
| Note<br />
|-<br />
| pCMV5&nbsp;<br />
| many<br />
| style="width: 178px;" | CMV5 promoter<br />
| style="width: 223px;" | CMV5-seq-F<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pEPD<br/><br />
| 3200<br />
| style="width: 178px;" | based on pADL-23c<br />
| style="width: 223px;" | pADL23C-seq-F CCAGGCTTTACACTTTATGCTTC<br/><br />
| <br/><br />
| pADL23C-seq-R GTTGAAAGTTGTTTAGCAAAACC<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pADL-23c<br/><br />
| 3092<br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | pADL23C-seq-F&nbsp;<br/><br />
| <br/><br />
| pADL23C-seq-R&nbsp;<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pT7A3<br />
| 1279<br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | pT7A3-seq-F, [[Sequencing_of_Difficult_template|difficult template]]<br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pT7A30<br />
| 1727<br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | pT7A3-seq-F, [[Sequencing_of_Difficult_template|difficult template]]<br/><br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pET28b<br />
| <br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | T7 F<br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pET30b<br />
| <br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | T7 F<br/><br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pT7A30-6His-pMBP<br/><br />
| 947<br/><br />
| style="width: 178px;" | 6His-pMBP<br/><br />
| style="width: 223px;" | MBP-seq-F<br/><br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pT7A30-pMBP<br/><br />
| 945&nbsp;&nbsp;<br/><br />
| style="width: 178px;" | pMBP<br />
| style="width: 223px;" | MBP-seq-F<br/><br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pCold-MBP-(3C)<br/><br />
| 1611<br/><br />
| style="width: 178px;" | 8XHis-MBP-(3C)<br/><br />
| style="width: 223px;" | <br />
MBP-seq-F<br />
<br />
GATGAAGCCCTGAAAGACGCGCAG<br />
<br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pCold-MBP-(E3C)<br/><br />
| 1804&nbsp;&nbsp;<br/><br />
| style="width: 178px;" | MBP-(E3C)<br />
| style="width: 223px;" | MBP-seq-F<br/><br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pCold-6H-(TEV)-MBP-(3C)<br/><br />
| 1756<br/><br />
| style="width: 178px;" | 6XHis-MBP-(E3C)<br/><br />
| style="width: 223px;" | MBP-seq-F<br/><br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pCold-MBP-(a3C)-MCS-3C<br/><br />
| 1807&nbsp;&nbsp;<br/><br />
| style="width: 178px;" | MBP-(a3C)-MCS-3C<br/><br />
| style="width: 223px;" | MBP-seq-F<br/><br />
| <br/><br />
| &nbsp;N/A<br />
| <br/><br />
| <br/><br />
|-<br />
| pCold-6His-MCS-(TEV-3C)-MBP<br/><br />
| 1798<br/><br />
| style="width: 178px;" | 6His-MCS-(TEV-3C)-MBP<br/><br />
| style="width: 223px;" | pCold-seq-F<br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pCold-GST-(3C)<br/><br />
| 1629<br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | GST-seq-F<br/><br />
| 2012_5_21 B11<br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pCold-8XHis-MBP-(3C)-MCS<br/><br />
| 1659&nbsp;<br/><br />
| style="width: 178px;" | 8His-MBP-(3C)<br />
| style="width: 223px;" | MBP-seq-F<br/><br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| &nbsp;pCold-7His-GST-(E)<br/><br />
| 1612<br/><br />
| style="width: 178px;" | 7His-GST-(E)<br />
| style="width: 223px;" | GST-seq-F<br/><br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pCold-6His-GST-(E)-(3C)<br/><br />
| 1624<br/><br />
| style="width: 178px;" | 6His-GST-(E)-(3C)<br/><br />
| style="width: 223px;" | GST-seq-F<br/><br />
| <br/><br />
| T7 R (T7 Term)<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pET28-cold<br/><br />
| v19&nbsp;<br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | pCold-seq-F<br/><br />
| <br/><br />
| pCold-seq-R<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pCold-6H<br />
| 1755<br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | pCold-seq-F AGCACGCCATATCGCCGAAAG<br/><br />
| 3_11_2013 B05<br/><br />
| <br />
pCold-seq-R GGCAGGGATCTTAGATTCTG or T7 R (T7 Term)<br />
<br />
| 3_11_2013 B06<br/><br />
| <br/><br />
|-<br />
| pCold-TF-(E3C)-3C<br/><br />
| 1729<br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| &nbsp;&nbsp;<br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| <br/><br />
| <br/><br />
| style="width: 178px;" | <br/><br />
| style="width: 223px;" | <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pEF1-HTLV-1IFH-HA-VH-mIgG2b<br/><br />
| 3165<br/><br />
| style="width: 178px;" | <br />
hEF1-HTLV-seq-F&nbsp; CTCCCTTGGAGCCTACCTAG<br />
<br />
EF1-HTLV-seq-F CTGCTTGCTCAACTCTACGTC<br />
<br />
| style="width: 223px;" | 2012_5_15 A06 ,&nbsp;9/14/17<br/><br />
| <br/><br />
| pFuse-seq-R GCATTCTAGTTGTGGTTTGTCC<br/><br />
| <br/><br />
| <br/><br />
|-<br />
| pCMV-HA-VH-mIgG2b<br/><br />
| 3166<br/><br />
| style="width: 178px;" | CMV-F<br />
| style="width: 223px;" | (Genscript)<br />
| <br/><br />
| <br/><br />
| <br/><br />
| <br/><br />
|-<br />
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| style="width: 178px;" | <br/><br />
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[[Category:To Do]] [[Category:Tasks]] [[Category:My ELN]]</div>
Crm