Paul.xu at 21:37, 24 December 2017

2017-12-24T21:37:23Z

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{| border="1" cellspacing="1" cellpadding="1" style="width: 647px;"
|-
| style="width: 51px;" | ID
| style="width: 85px;" | Name
| style="width: 69px;" | Promoter
| style="width: 19px;" | Signal
| style="width: 60px;" | Species
| style="width: 55px;" | Isotype
| style="width: 130px;" | MCS
| style="width: 25px;" | Status
| style="width: 115px;" | Note
|-
| style="width: 51px;" | 2492 
| style="width: 85px;" | pCMV5-mIgG2b-HC 
| style="width: 69px;" | CMV5
| style="width: 19px;" | none
| style="width: 60px;" | mouse
| style="width: 55px;" | IgG2b Heavy 
| style="width: 130px;" |
BamH1 GGATCC-- Kpn1 GGTACC

GT/AKTTAPS

| style="width: 25px;" | 0
| style="width: 115px;" | not compatible with VH domain designed for pCEH0
|-
| style="width: 51px;" |  2493 
| style="width: 85px;" | pCMV5-mIgG-kLC 
| style="width: 69px;" | CMV5
| style="width: 19px;" | none
| style="width: 60px;" | mouse 
| style="width: 55px;" | kappa light 
| style="width: 130px;" |
BamH1 GGATCC-- Kpn1 GGTACC

GT/ADAAP

| style="width: 25px;" | 0
| style="width: 115px;" | not compatible with VL domain designed for pCEL0 
|-
| style="width: 51px;" | 3016 
| style="width: 85px;" | pCEL0 
| style="width: 69px;" | CAG
| style="width: 19px;" |
yes

| style="width: 60px;" | 
| style="width: 55px;" |  light
| style="width: 130px;" |
BssH2                   Nhe1

GCGCGC TGT ---- GCTAGC

| style="width: 25px;" | 0
| style="width: 115px;" |
backbone

Nhe1 is compatible with Xba1

|-
| style="width: 51px;" | 3017 
| style="width: 85px;" |  pCEH0 
| style="width: 69px;" | CAG
| style="width: 19px;" | yes
| style="width: 60px;" | 
| style="width: 55px;" | heavy
| style="width: 130px;" |
BsrG1

TGTaCActcc--Sal1,  Kpn1, pvUII, Nhe1, HindIII, Not1

| style="width: 25px;" | 0
| style="width: 115px;" | backbone 
|-
| style="width: 51px;" | 3020 
| style="width: 85px;" | pCEH0-mIgG1-HC 
| style="width: 69px;" | CAG
| style="width: 19px;" | yes
| style="width: 60px;" | mouse
| style="width: 55px;" |
mIgG1

heavy

| style="width: 130px;" |
BsrG1   Nhe1 TGTaCActcc ---GCTAGC 

ASSAKTTPP

| style="width: 25px;" | 1
| style="width: 115px;" | VH domain
|-
| style="width: 51px;" | 3021 
| style="width: 85px;" | pCEL0-mIg-LCKappa 
| style="width: 69px;" | CAG
| style="width: 19px;" | yes
| style="width: 60px;" | mouse
| style="width: 55px;" |
Ig

Kappa

| style="width: 130px;" |
BssHII             KpnI or Xho1 

GCGCGCTGT-- GGtACC

GT/KLEIKRADAAP

| style="width: 25px;" | 1
| style="width: 115px;" | VH
|-
| style="width: 51px;" | 3025 
| style="width: 85px;" | pCEL0-rabbit-Ig-LKappa 
| style="width: 69px;" | CAG
| style="width: 19px;" | yes
| style="width: 60px;" | rabbit
| style="width: 55px;" | light kappa
| style="width: 130px;" |
BssHII  --Kpn1

GCGCGC -- GGtACC

GT/E V V V K G D

| style="width: 25px;" | in pJet1.2 now, need to sublcone
| style="width: 115px;" | 
|-
| style="width: 51px;" | 3026  
| style="width: 85px;" | pCEH0-rabbit-IgG-HC 
| style="width: 69px;" | CAG
| style="width: 19px;" | yes
| style="width: 60px;" | rabbit
| style="width: 55px;" | heavy
| style="width: 130px;" |
BsrG1--Nhe1

GCTAGC

AS/SGQPKAP

| style="width: 25px;" | in pJet1.2 now, need to sublcone 
| style="width: 115px;" | 
|-
| style="width: 51px;" | 3027 
| style="width: 85px;" | pCEH0-mIgG2b-HC 
| style="width: 69px;" | CAG
| style="width: 19px;" | yes
| style="width: 60px;" | mouse
| style="width: 55px;" | IgG2b
| style="width: 130px;" |
BsrG1--Nhe1

AS/SAKTTPP

| style="width: 25px;" | need to PCR from 2492
| style="width: 115px;" | 
|-
| style="width: 51px;" | pFuse4 
| style="width: 85px;" | pFUSE-hIgG4-Fc1 
| style="width: 69px;" | hEF1-HTLV 
| style="width: 19px;" | yes
| style="width: 60px;" | human
| style="width: 55px;" | IgG4
| style="width: 130px;" | BamH1--Xba1
| style="width: 25px;" | 0
| style="width: 115px;" | 
|-
| style="width: 51px;" | 3030 
| style="width: 85px;" | pCEH0-mIgG2a-HC 
| style="width: 69px;" | cag
| style="width: 19px;" | yes
| style="width: 60px;" | mouse
| style="width: 55px;" | mIgG2a 
| style="width: 130px;" |
BsrG1--Nhe1

AS/SAKTTPP

| style="width: 25px;" | need to find mouse IgG2a clone
| style="width: 115px;" | 
|-
| style="width: 51px;" | 3096 
| style="width: 85px;" | pCEL0-L-clone3 
| style="width: 69px;" | CAG
| style="width: 19px;" | yes
| style="width: 60px;" | human
| style="width: 55px;" | light kappa 
| style="width: 130px;" |
BssHII--Nhe1            AgeI  (entire kappa)

| style="width: 25px;" | 
| style="width: 115px;" | 
|-
| style="width: 51px;" | 3095 
| style="width: 85px;" | pCEH0-H-clone3 
| style="width: 69px;" | cag
| style="width: 19px;" | yes
| style="width: 60px;" | human
| style="width: 55px;" | heavy
| style="width: 130px;" | BsrG1 
| style="width: 25px;" | 
| style="width: 115px;" | 
|-
| style="width: 51px;" | 
| style="width: 85px;" | 
| style="width: 69px;" | 
| style="width: 19px;" | 
| style="width: 60px;" | 
| style="width: 55px;" | 
| style="width: 130px;" | 
| style="width: 25px;" | 
| style="width: 115px;" | 
|}
[[Category:Antibodies]]

Vectors for Antibody Expression - Revision history

Paul.xu at 21:37, 24 December 2017