Paul.xu at 19:23, 1 April 2018

2018-04-01T19:23:06Z

New page

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Status
| Clone ID
| Parent
| Name
| Note
| Ref
|-
| 1
| 3127   
| 3097  
| pET28b-qBeta-qBeta-6XHis 
| 1st copy: BamH1-Xho1, no his tag; 2nd copy to be cloned, with c_his tag
| 
|-
| 0
| 3097
| 
| 
| 
| 
|-
| 1
| 971 
| 
| pT7A3-SpyCatcher-AP205cp3 
| AP205cp3 
| 
|-
| 0
| 3098  
| 
| pET28b-AP205 
| AP205, Nco1 + Kpn1 site for insertion of peptide <15 aa at N-terminus, 6XHis tag at C-termnus, up to 55 aa at C-termunus 
| [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2843720 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2843720] 
|-
| 0 
| 3105 
| 
| pET28b-AaLS
| AaLs 
| 
|-
| 
| 
| 
| PP7
| 
| / 
|-
| 
| 
| 
| MS2 
| 
| 
|}

{| border="1" cellspacing="1" cellpadding="1" style="width: 736px;"
|-
| Status
| ID
| Name
| Plasmid Name
| style="width: 8px;" | Host 
| style="width: 4px;" | Expression
| style="width: 292px;" | Note
|-
| 1
| 985 
| M13-g3p
| pCold-GST-3C-AP205-M13-g3p 
| style="width: 8px;" | 
| style="width: 4px;" | 
| style="width: 292px;" | waiting for subclone 
|-
| 2
| 973 
| M13-g3p 
| pT7A3-6H-SBP-SUMO-3C-pCold-GST-3C-AP205-M13-g3p 
| style="width: 8px;" | 
| style="width: 4px;" | 
| style="width: 292px;" | waiting for subclone 
|-
| 0
| 982 
| 
| pMiniT2-973-1 
| style="width: 8px;" | 
| style="width: 4px;" | 
| style="width: 292px;" | 6His-SBP-SUMO-3C-Myc, Flag, V5, M13 gene VIII,  gene 3 , HA, His 
|-
| 1
| 983 
| 
| pMiniT2-973-2 
| style="width: 8px;" | 
| style="width: 4px;" | 
| style="width: 292px;" | Ap205, M13 gene 3 
|-
| 1
| 3127 
| 
| pET28b-qBeta-qBeta-6XHis 
| style="width: 8px;" | 
| style="width: 4px;" | 
| style="width: 292px;" | VLP, M13 Phage GeneVIII 
|-
| 0
| 3105 
| 
| pET28b-AaLS 
| style="width: 8px;" | 
| style="width: 4px;" | 
| style="width: 292px;" | Thermostable Lumazine Synthase From Aquifex Aeolicus, VLP, Gene VIII 
|-
| 0
| 3098 
| 
| pET28b-AP205 
| style="width: 8px;" | 
| style="width: 4px;" | 
| style="width: 292px;" | Acinetobacter phage  major capsid protein AP205, VLP, M13 gene VIII 
|}

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[[Category:Antibodies]] [[Category:Tasks]] [[Category:To Do]]

Virus Like Particles - Revision history

Paul.xu at 19:23, 1 April 2018