Paul.xu: Created page with "Pick a single colony Saccharomyces cerevisiae from a YPD plate Suspend in 100 μl of 200 mM LiOAc 1% SDS solution Incubate at 70°C for 15 minutes. Add 300 μl of 96..."

2020-06-01T15:10:20Z

Created page with "Pick a single colony Saccharomyces cerevisiae from a YPD plate Suspend in 100 μl of 200 mM LiOAc 1% SDS solution Incubate at 70°C for 15 minutes. Add 300 μl of 96..."

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Pick a single colony Saccharomyces cerevisiae from a YPD plate

Suspend in 100 μl of 200 mM LiOAc 1% SDS solution

Incubate at 70°C for 15 minutes.

Add 300 μl of 96% ethanol

Spin 15, 000 g for 3 minutes

Dissolve DNA in 100 μl of TE

Spin down cell debris for 15 000 g; 1 minute

Use 1 μl of the supernatant  for PCR
[[Category:Protocols]]

Yeast Genomic DNA Extraction - Revision history

Paul.xu: Created page with "Pick a single colony Saccharomyces cerevisiae from a YPD plate Suspend in 100 μl of 200 mM LiOAc 1% SDS solution Incubate at 70°C for 15 minutes. Add 300 μl of 96..."