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	<title>Yidan 2016 10 04 - Revision history</title>
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	<updated>2026-10-18T03:12:46Z</updated>
	<subtitle>Revision history for this page on the wiki</subtitle>
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	<entry>
		<id>https://eln.excellgen.com/index.php?title=Yidan_2016_10_04&amp;diff=15403&amp;oldid=prev</id>
		<title>Crm at 21:24, 4 October 2016</title>
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		<updated>2016-10-04T21:24:10Z</updated>

		<summary type="html">&lt;p&gt;&lt;/p&gt;
&lt;p&gt;&lt;b&gt;New page&lt;/b&gt;&lt;/p&gt;&lt;div&gt;&amp;#039;&amp;#039;&amp;#039;Trec PCR Screening&amp;#039;&amp;#039;&amp;#039;&lt;br /&gt;
&lt;br /&gt;
1 ul of 10 uM primers for 25 ul PCR, 1 ul 10 mM dNTP&amp;lt;br/&amp;gt;0.5ul Taq&lt;br /&gt;
&lt;br /&gt;
PCR cycle:&lt;br /&gt;
&lt;br /&gt;
1 min at 94 oC&lt;br /&gt;
&lt;br /&gt;
35 cycles:&amp;lt;br/&amp;gt;30 s at 94 oC&amp;lt;br/&amp;gt;30 s at 55 oC&amp;lt;br/&amp;gt;1 min at 72 oC&lt;br /&gt;
&lt;br /&gt;
5 min at 72 oC&lt;br /&gt;
&lt;br /&gt;
[[File:IMG 1689.JPG|RTENOTITLE]]&lt;br /&gt;
[[Category:My ELN]]&lt;/div&gt;</summary>
		<author><name>Crm</name></author>
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