https://eln.excellgen.com/index.php?action=history&feed=atom&title=Yidan_2016_10_06 2026-10-18T03:12:32Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Yidan_2016_10_06&diff=15420&oldid=prev 2016-10-10T19:34:48Z

<p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Purpose:&#039;&#039;&#039; Screening of different taq.<br /> <br /> &#039;&#039;&#039;Procedure:&#039;&#039;&#039;<br /> <br /> Apply each taq to two Ni columns.<br /> <br /> First Ni column: Lyse, centrifuge down, apply supernatant to Ni column.<br /> <br /> Second Ni column: Add lysis buffer to the pellet again, centrifuge down. Appply supernatant to another Ni column.<br /> <br /> &#039;&#039;&#039;Result:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;[[File:Taq Screening.jpg]]&#039;&#039;&#039;<br /> <br /> {| style=&quot;width: 517px&quot; border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | style=&quot;text-align: center&quot; | 1&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 2&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 3&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 4&lt;br/&gt;<br /> | style=&quot;width: 43px; text-align: center&quot; | 5&lt;br/&gt;<br /> | style=&quot;width: 44px; text-align: center&quot; | 6&lt;br/&gt;<br /> | style=&quot;width: 41px; text-align: center&quot; | 7&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 8&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 9&lt;br/&gt;<br /> | style=&quot;width: 49px; text-align: center&quot; | 10&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 251px; text-align: center&quot; colspan=&quot;5&quot; rowspan=&quot;1&quot; | Second Ni column&lt;br/&gt;<br /> | style=&quot;width: 249px; text-align: center&quot; colspan=&quot;5&quot; rowspan=&quot;1&quot; | First Ni column&lt;br/&gt;<br /> |-<br /> | style=&quot;text-align: center&quot; | 2080&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 2040&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 2039&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 1032&lt;br/&gt;<br /> | style=&quot;width: 43px; text-align: center&quot; | 512&lt;br/&gt;<br /> | style=&quot;width: 44px; text-align: center&quot; | 2080&lt;br/&gt;<br /> | style=&quot;width: 41px; text-align: center&quot; | 2040&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 2039&lt;br/&gt;<br /> | style=&quot;text-align: center&quot; | 1032&lt;br/&gt;<br /> | style=&quot;width: 49px; text-align: center&quot; | 512&lt;br/&gt;<br /> |}<br /> <br /> [[Category:My ELN]]</div>

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