https://eln.excellgen.com/index.php?action=history&feed=atom&title=Yidan_2016_9_27 2026-10-18T03:12:46Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Yidan_2016_9_27&diff=15377&oldid=prev 2016-09-30T18:22:23Z

<p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Name:&#039;&#039;&#039; Yidan Hu<br /> <br /> &#039;&#039;&#039;Date&#039;&#039;&#039;: Sept 27, 2016<br /> <br /> == EXP1 ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039;&amp;nbsp;Find out if home-made&amp;nbsp;Nde1 enzyme is still good for plasmid DNA digestion<br /> <br /> &#039;&#039;&#039;Procedure:&#039;&#039;&#039;<br /> <br /> Digest 0.5ug plasmid 2411. Add 1ul of NdeI.<br /> <br /> &#039;&#039;&#039;Result:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;[[File:Nde1 digestion.JPG|RTENOTITLE]]&#039;&#039;&#039;<br /> <br /> {| style=&quot;width: 500px&quot; border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> | 1&lt;br/&gt;<br /> | 2&lt;br/&gt;<br /> | 3&lt;br/&gt;<br /> | 4&lt;br/&gt;<br /> | 5&lt;br/&gt;<br /> | 6&lt;br/&gt;<br /> | 7&lt;br/&gt;<br /> | 8&lt;br/&gt;<br /> | 9&lt;br/&gt;<br /> | 10&lt;br/&gt;<br /> | 11&lt;br/&gt;<br /> | 12&lt;br/&gt;<br /> | 13&lt;br/&gt;<br /> | 14&lt;br/&gt;<br /> |-<br /> | <br /> digestion<br /> <br /> enzyme<br /> <br /> | &lt;br/&gt;<br /> | HindIII&lt;br/&gt;<br /> | <br /> NdeI<br /> <br /> 25ug/ml<br /> <br /> | <br /> NdeI<br /> <br /> 50ug/ml<br /> <br /> | <br /> NdeI<br /> <br /> 100ug/ml<br /> <br /> | <br /> NdeI<br /> <br /> 200ug/ml<br /> <br /> | <br /> NdeI<br /> <br /> 400ug/ml<br /> <br /> | <br /> NdeI(old)<br /> <br /> 100ug/ml<br /> <br /> | <br /> HindIII+<br /> <br /> NdeI(25ug/ml)<br /> <br /> | <br /> HindIII+<br /> <br /> NdeI(50ug/ml)<br /> <br /> | <br /> HindIII+<br /> <br /> NdeI(100ug/ml)<br /> <br /> | <br /> HindIII+<br /> <br /> NdeI(200ug/ml)<br /> <br /> | <br /> HindIII+<br /> <br /> NdeI(400ug/ml)<br /> <br /> | <br /> HindIII+<br /> <br /> NdeI(old,100ug/ml)<br /> <br /> |}<br /> <br /> == &#039;&#039;&#039;EXP 2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose: &#039;&#039;&#039; 2477&amp;nbsp;pEC2-ALB, 2478&amp;nbsp;pEC2-betaActin,2482&amp;nbsp;pEC2-maize-actin1<br /> <br /> &#039;&#039;&#039;Procedure: Make miniprep and digest with HindIII and NdeI.&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Result:&#039;&#039;&#039;<br /> <br /> 2477&amp;nbsp;pEC2-ALB<br /> <br /> &#039;&#039;&#039;[[File:ALB digestion.JPG|RTENOTITLE]]&#039;&#039;&#039;<br /> <br /> 2478&amp;nbsp;pEC2-betaActin<br /> <br /> [[File:BetaActin digestion.JPG|RTENOTITLE]]<br /> <br /> 2482&amp;nbsp;pEC2-maize-actin1<br /> <br /> [[File:Maize actin digestion.JPG|RTENOTITLE]]<br /> [[Category:My ELN]]</div>

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