https://eln.excellgen.com/index.php?action=history&feed=atom&title=Yidan_2016_9_28 Yidan 2016 9 28 - Revision history 2026-10-18T02:16:01Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Yidan_2016_9_28&diff=15396&oldid=prev Crm at 18:49, 30 September 2016 2016-09-30T18:49:02Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;EXP1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose: PCR of 2489&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Procedure:&#039;&#039;&#039;<br /> <br /> Template: Trec-combo<br /> <br /> Primer: 2477-ALB-NdeI-F, 2478-betaActin-XhoI-R<br /> <br /> PCR cycle:<br /> <br /> 1 min at 94 oC<br /> <br /> 30 cycles:&lt;br/&gt;30 s at 94 oC&lt;br/&gt;30 s at 55 oC&lt;br/&gt;1.5 min at 72 oC<br /> <br /> 5 min at 72 oC<br /> <br /> &#039;&#039;&#039;Result:&#039;&#039;&#039;<br /> <br /> [[File:IMG 1680.JPG|RTENOTITLE]]<br /> <br /> [[File:IMG 1683.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;EXP2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; PCR of maize actin<br /> <br /> &#039;&#039;&#039;Procedure:&#039;&#039;&#039;<br /> <br /> Template: Maize genomic DNA<br /> <br /> Primer: 2482-maize-Actin1-Nde1-F, 2482-maize-Actin1-Xho1-R<br /> <br /> PCR cycle:<br /> <br /> 1 min at 94 oC<br /> <br /> 30 cycles:&lt;br/&gt;30 s at 94 oC&lt;br/&gt;30 s at 55 oC&lt;br/&gt;30 s at 72 oC<br /> <br /> 5 min at 72 oC<br /> <br /> &#039;&#039;&#039;Result:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;[[File:IMG 1677.JPG|RTENOTITLE]]&#039;&#039;&#039;<br /> [[Category:My ELN]]</div> Crm