https://eln.excellgen.com/index.php?action=history&feed=atom&title=Yidan_2016_9_29 Yidan 2016 9 29 - Revision history 2026-10-18T03:12:45Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Yidan_2016_9_29&diff=15386&oldid=prev Crm at 18:34, 30 September 2016 2016-09-30T18:34:46Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;EXP1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose: &#039;&#039;&#039;2489<br /> <br /> &#039;&#039;&#039;Procedure:&#039;&#039;&#039;<br /> <br /> Make miniprep and digest with EcoRI.<br /> <br /> PCR Screening<br /> <br /> &#039;&#039;&#039;Result:&#039;&#039;&#039;<br /> <br /> Digest with EcoRI<br /> <br /> &#039;&#039;&#039;[[File:2489 digestion.JPG|RTENOTITLE]]&#039;&#039;&#039;<br /> <br /> PCR screening<br /> <br /> Use forward primer and reverse primer from Biolabs.<br /> <br /> [[File:2489 PCR screening.JPG|RTENOTITLE]]<br /> <br /> Send sample 1 and 5 for sequencing.<br /> <br /> == &#039;&#039;&#039;EXP2&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose: PCR using Trec-combo template and Sample 1 in EXP1.&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Procedure:&#039;&#039;&#039;<br /> <br /> Taq: Lot 67-2038 PCR, 0.5ul.<br /> <br /> PCR cycle:<br /> <br /> 1 min at 94 oC<br /> <br /> 30 cycles:&lt;br/&gt;30 s at 94 oC&lt;br/&gt;30 s at 55 oC&lt;br/&gt;1 min at 72 oC<br /> <br /> 5 min at 72 oC<br /> <br /> &#039;&#039;&#039;Result:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;[[File:PCR comparison.JPG|RTENOTITLE]]&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot; width=&quot;390&quot;<br /> |-<br /> | style=&quot;width: 39px&quot; | &lt;br/&gt;<br /> | style=&quot;width: 256px&quot; | 1&lt;br/&gt;<br /> | style=&quot;width: 256px&quot; | 2&lt;br/&gt;<br /> | style=&quot;width: 256px&quot; | 3&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 39px&quot; | Template&lt;br/&gt;<br /> | style=&quot;width: 256px&quot; | Trec-combo&lt;br/&gt;<br /> | style=&quot;width: 256px&quot; | Sample 1 in EXP1&lt;br/&gt;<br /> | style=&quot;width: 256px&quot; | Sample 1 in EXP1&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 39px&quot; | Primer&lt;br/&gt;<br /> | style=&quot;width: 256px&quot; | 2477-ALB-NdeI-F, 2478-betaActin-XhoI-R&lt;br/&gt;<br /> | style=&quot;width: 256px&quot; | 2477-ALB-NdeI-F, 2478-betaActin-XhoI-R&lt;br/&gt;<br /> | style=&quot;width: 256px&quot; | Primer from Biolabs&lt;br/&gt;<br /> |}<br /> [[Category:My ELN]]</div> Crm