https://eln.excellgen.com/index.php?action=history&feed=atom&title=Yidan_2016_9_30 2026-10-18T03:12:36Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Yidan_2016_9_30&diff=15375&oldid=prev 2016-09-30T17:45:48Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;EXP1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Comparison of different Taq<br /> <br /> &#039;&#039;&#039;Procedure:&#039;&#039;&#039;<br /> <br /> Template: Trec combo(150664295) <br /> <br /> Primer: 2477-ALB-NdeI-F, 2478-betaActin-XhoI-R<br /> <br /> 1 ul of 10 uM primers for 25 ul PCR, 1 ul 10 mM dNTP&lt;br/&gt;0.5ul Taq<br /> <br /> PCR cycle:<br /> <br /> 1 min at 94 oC<br /> <br /> 30 cycles:&lt;br/&gt;30 s at 94 oC&lt;br/&gt;30 s at 55 oC&lt;br/&gt;1 min at 72 oC<br /> <br /> 5 min at 72 oC<br /> <br /> &#039;&#039;&#039;Result:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;[[File:IMG 1687.JPG|RTENOTITLE]]&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;PCR product is 1kb&#039;&#039;&#039;<br /> <br /> {| style=&quot;width: 336px&quot; border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | style=&quot;width: 39px; text-align: center&quot; | 1&lt;br/&gt;<br /> | style=&quot;width: 281px; text-align: center&quot; | <br /> &amp;nbsp;invitrogen Taq DNA polymerase<br /> <br /> Lot:1329498 Exp: 03/2008<br /> <br /> |-<br /> | style=&quot;width: 39px; text-align: center&quot; | 2&lt;br/&gt;<br /> | style=&quot;width: 281px; text-align: center&quot; | &amp;nbsp;Qiagen Hot start taq&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 39px; text-align: center&quot; | 3&lt;br/&gt;<br /> | style=&quot;width: 281px; text-align: center&quot; | &amp;nbsp;Biolabs One Taq Hot Start DNA polymerase Lot:0011101 Exp:01/2013&lt;br/&gt;<br /> |}<br /> [[Category:My ELN]]</div>

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