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Yijia LI 2 28 2018 - Revision history
2026-10-18T06:26:38Z
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Crm at 18:50, 1 March 2018
2018-03-01T18:50:27Z
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<p><b>New page</b></p><div>== Date:2/28/2018 ==<br />
<br />
Miniprep digestion for 3154_3129<br />
<br />
Digestion buffer:Fastdigest<br />
<br />
Restriction enzymes:<br />
<br />
3154:BamH I+ Kpn I'''insert length:527'''<br />
<br />
3129:Kpn I+Not I'''inset length:1034'''<br />
<br />
[[File:Miniprep digestion for 3154 3129.JPG|RTENOTITLE]]<br />
<br />
Result:seems good, chose 3129-1 to do sequencing<br />
<br />
== Date:2/27/2018 ==<br />
<br />
pCEL0<br />
<br />
[[File:Miniprep of 3027 3021.JPG|RTENOTITLE]]<br />
<br />
== Date:2/28/1028 ==<br />
<br />
Digestion of 3129 vector with two enzymes:<br />
<br />
BsrG I+Kpn I,&nbsp;BsrG I only,&nbsp;Kpn only<br />
<br />
Buffer NEB 2<br />
<br />
&nbsp;Volume of vector:0.5 ul<br />
<br />
[[File:3129 digestion test.JPG|RTENOTITLE]]<br />
<br />
== ==<br />
<br />
== Date:3/1/2018 ==<br />
<br />
'''Test for 3129-pCMV5-HA-VH-mIgG2b digestion sites:'''<br/>1:Sac I +BsrG I<br/>2:Sall+BsrG I<br/>3:Xho I+ BsrG I<br/>4:BsrG I<br/>5:Kpn I+BsrG I<br/>Volume of DNA:1 ul<br/>Buffer:Fastdigest&nbsp;<br/>Time:30mins<br />
<br />
[[File:Digestion test for 3129.JPG|RTENOTITLE]]<br />
[[Category:My ELN]]</div>
Crm