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Yijia LI 3 26 2018 - Revision history
2026-10-18T13:21:30Z
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Crm at 00:40, 5 April 2018
2018-04-05T00:40:24Z
<p></p>
<p><b>New page</b></p><div>== Date:3/26/2018 ==<br />
<br />
'''Subclone of 947'''<br />
<br />
Vector:1727<br />
<br />
Insert:947pMiniT2---#3<br />
<br />
XbaI+NcoI-HF<br />
<br />
[[File:IMG 3647.JPG|RTENOTITLE]]<br />
<br />
'''Subclone of 945'''<br />
<br />
Vector:1727<br />
<br />
Insert:945pMiniT2<br />
<br />
XbaI+NcoI-HF<br />
<br />
[[File:IMG 3651.JPG|RTENOTITLE]]<br />
<br />
'''Test of 947_945_3166_3165'''<br />
<br />
{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| Name<br />
| Enzymes<br />
| Size (bp)<br />
| Lane<br />
|-<br />
| 947<br />
| BglII<br />
| 646<br />
| 1,2,3<br />
|-<br />
| <br/><br />
| BssHII<br />
| 1856<br />
| 4,5,6<br />
|-<br />
| 945<br />
| BglII<br />
| 628<br />
| 11,12<br />
|-<br />
| <br/><br />
| BssHII<br />
| 1838<br />
| 13<br />
|-<br />
| 3165<br />
| SalI+BsrGI<br />
| 693<br />
| 8<br />
|-<br />
| <br/><br />
| HindIII+BsrGI<br />
| 441<br />
| 10<br />
|-<br />
| 3166<br />
| SalI+BsrGI<br />
| 1234<br />
| 9<br />
|-<br />
| <br/><br />
| XbaI<br />
| 2049<br />
| 7<br />
|-<br />
| <br/><br />
| BglII<br />
| 3259<br />
| to do<br />
|}<br />
<br />
[[File:IMG 36521.JPG|RTENOTITLE]]<br />
<br />
== ==<br />
<br />
'''PCR of 3127'''<br />
<br />
3127 PCR<br />
<br />
3097 as template<br />
<br />
3127-NdeI-F<br />
<br />
3127-HindIII-R<br />
<br />
408 bp<br />
<br />
extension:30s<br />
<br />
[[File:IMG 3654.JPG|RTENOTITLE]]<br />
<br />
'''Subclone of 3165'''<br />
<br />
Vector:3129<br />
<br />
Insert:3165pMiniT2<br />
<br />
SalI+BsrGI<br />
<br />
[[File:IMG 3649.JPG|RTENOTITLE]]<br />
<br />
== Date:3/27/2018 ==<br />
<br />
'''Test of miniprep digestion'''<br />
<br />
3166: lane 1-3, SalI+BsrGI 1234bp<br />
<br />
3166: lane 4-6, XbaI 2049bp<br />
<br />
3166: lane 7-9, BglII 3259bp<br />
<br />
3165: lane 10,11, SalI+BsrGI 693bp<br />
<br />
3165: lane 12,13, HindIII+BsrGI 441bp<br />
<br />
[[File:IMG 3660.JPG|RTENOTITLE]]<br />
<br />
'''redo 3152 subclone'''<br />
<br />
[[File:IMG 3669.JPG|RTENOTITLE]]<br />
<br />
'''Subclone of 3122'''<br />
<br />
[[File:IMG 3670.JPG|RTENOTITLE]]<br />
<br />
'''subclone of 939'''<br />
<br />
[[File:IMG 3675.JPG|RTENOTITLE]]<br />
<br />
'''subclone of 938'''<br />
<br />
Size:1145bp<br />
<br />
V:1987 pT7A3<br />
<br />
[[File:IMG 3672.JPG]]<br />
<br />
[[Category:My ELN]]<br />
<br />
== Date:3/28/2018 ==<br />
<br />
[[File:Miniprep digestion of 947 945 3165 03282018.JPG|RTENOTITLE]]<br />
<br />
== Date:3/29/2018 ==<br />
<br />
'''Miniprep of 3152 digestion'''<br />
<br />
1-4:samples<br />
<br />
+:control for 3009pminiT2<br />
<br />
Enzymes:BsrGI+NheI<br />
<br />
[[File:IMG 3684.JPG|RTENOTITLE]]<br />
<br />
'''Miniprep of 3127p_3166_973-2_973-1 digestion'''<br />
<br />
3127pMiniT2:1-4<br />
<br />
3166pMiniT2:5-8<br />
<br />
973-2pMiniT2:9-12<br />
<br />
973-1pMiniT2:13-16<br />
<br />
Enzymes:EcoRI<br />
<br />
3166 itself has EcoRI sites<br />
<br />
[[File:IMG 3685.JPG|RTENOTITLE]]<br />
<br />
== Date:3/30/2018 ==<br />
<br />
'''Miniprep digestion of 3122_938_939'''<br />
<br />
3122:lane 1-4 lane 5&nbsp;:+ size:1743 bp<br />
<br />
938: lane 6-9 lane 10:+ size:1145 bp<br />
<br />
939: lane 11-14 lane 15:+ size:780 bp<br />
<br />
Positive control are insert-pjet1.2<br />
<br />
The small band size of vector 1727:193 bp<br />
<br />
[[File:IMG 0535.JPG|RTENOTITLE]]<br />
<br />
'''redo Miniprep digestion of 3122_938_939'''<br />
<br />
1:3122&nbsp; size:1743bp<br />
<br />
2:3122pjet1.2 sequenced +<br />
<br />
3:938&nbsp; size:1145 bp<br />
<br />
4:938pjet1.2 sequenced +<br />
<br />
5,6,7:939&nbsp; size:780 bp<br />
<br />
8:939pjet1.2 sequenced +<br />
<br />
Enzymes:NdeI+XhoI<br />
<br />
[[File:IMG 0536.JPG|RTENOTITLE]]<br />
<br />
== Date:4/1/2018 ==<br />
<br />
'''Miniprep digestion of 973-2pMiniT2'''<br />
<br />
Enzymes:EcoRI<br />
<br />
Size:500bp<br />
<br />
[[File:IMG 0602.JPG|RTENOTITLE]]<br />
[[Category:My ELN]]</div>
Crm