Crm at 20:48, 20 April 2018

2018-04-20T20:48:22Z

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== Date:4/19/2018 ==

'''Miniprep digestion of 824_984_3122'''

824:1-5, 5 is sequenced + Vector:1518pET28b

984:6-10, 10 is sequenced + Vector:1987pT7A3

3122:11-15, 15 is sequenced + Vector:1727 pT7A30

'''Result:suspicious result need to repeat'''

[[File:IMG 0703.JPG|RTENOTITLE]]

'''Miniprep of 931'''

Enzymes:Nde1+Xho1

Size:2160bp

'''Result:good, but need to confirm with Nde1+HindIII.Nde1+Xho1.HindIII+Xho1'''

[[File:IMG 0704.JPG|RTENOTITLE]]

== Date:4/20/2018 ==

'''Miniprep of 3122'''

Enzymes:Nde1+Xho1

Sample:#1-4

Lane #5 is sequenced +

[[File:IMG 0705.JPG|RTENOTITLE]]

'''Miniprep of 931_824'''

{| border="1" cellspacing="1" cellpadding="1" style="width: 804px;"
|-
| #
| Lane
| control lane
| style="width: 256px;" | enzymes
| style="width: 44px;" | size
| style="width: 162px;" | note
|-
| 931--2
| 1-3
| F1--lane10,  F2--lane11
| style="width: 256px;" |
1-Nde1+Xho1;

2-Nde1+HindIII;

3-HindIII+Xho1

| style="width: 44px;" | 
| style="width: 162px;" | 
|-
| 931--3
| 4-6
| F1--lane10,  F2--lane11 
| style="width: 256px;" |
4-Nde1+Xho1;

5-Nde1+HindIII;

6-HindIII+Xho1

| style="width: 44px;" | 
| style="width: 162px;" | 
|-
| 931--4
| 7-9
| F1--lane10,  F2--lane11 
| style="width: 256px;" |
7-Nde1+Xho1;

8-Nde1+HindIII;

9-HindIII+Xho1

| style="width: 44px;" | 
| style="width: 162px;" | 
|-
| 824
| 12-15
| lane 16  832pMiniT2
| style="width: 256px;" | Nde1+Xho1
| style="width: 44px;" | 
| style="width: 162px;" | 
|}

[[File:IMG 0706.JPG|RTENOTITLE]]
[[Category:My ELN]]

Yijia LI 4 16 2018 - Revision history

Crm at 20:48, 20 April 2018