https://eln.excellgen.com/index.php?action=history&feed=atom&title=Yijia_LI_5_14_2018 2026-10-18T08:19:26Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Yijia_LI_5_14_2018&diff=17953&oldid=prev 2018-05-22T16:02:11Z

<p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Miniprep digestion of 3122_984_3192_3194&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Enzymes<br /> | Size<br /> | Note<br /> |-<br /> | 3122<br /> | 1-4<br /> | Nde1+Xho1<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 984<br /> | 5-8<br /> | Xba1+Nhe1<br /> | 957bp<br /> | Lane 8 maybe good,need to confirm the size<br /> |-<br /> | 3192<br /> | 9-12<br /> | Kpn1<br /> | &lt;br/&gt;<br /> | need to confirm the size<br /> |-<br /> | 3194<br /> | 13-16<br /> | Nhe1+Kpn1<br /> | &lt;br/&gt;<br /> | Maybe vector<br /> |}<br /> <br /> [[File:Miniprep dogestion of 3122 984 3192 3194.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 1000_3200_3166&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Enzymes<br /> | Size<br /> | Note<br /> |-<br /> | 1000<br /> | 1-3<br /> | HindIII+Xho1<br /> | 100<br /> | need to confirm the size<br /> |-<br /> | 3200<br /> | 4-6<br /> | BamH1+ECoR1<br /> | &lt;br/&gt;<br /> | no insert band should be good,redo<br /> |-<br /> | 3166<br /> | 7-10<br /> | Sal1+BsrG1&lt;br/&gt;<br /> | 1234<br /> | need to confirm the size<br /> |}<br /> <br /> [[File:Miniprep digestion of 1000 3200 3166.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 3191_931_3197&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | ENzymes<br /> | Size<br /> | Note<br /> |-<br /> | 3191<br /> | 1<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | Not good, redo<br /> |-<br /> | 931<br /> | 2,3<br /> | 2--HindIII+Nde1;3--HindIII+Xho1<br /> | both are around 900 bp<br /> | seems good, but lysis<br /> |-<br /> | 3197<br /> | 4,5<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | not good, lysis<br /> |}<br /> <br /> [[File:Miniprep digestin3191 931 3197.JPG|RTENOTITLE]]<br /> <br /> == Conclusion ==<br /> <br /> Potentially Good:984,3192,3166<br /> [[Category:My ELN]]</div>

Crm