https://eln.excellgen.com/index.php?action=history&feed=atom&title=Yijia_LI_5_29_2018 Yijia LI 5 29 2018 - Revision history 2026-10-18T08:19:17Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=Yijia_LI_5_29_2018&diff=18090&oldid=prev Crm at 23:06, 1 June 2018 2018-06-01T23:06:39Z <p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Vector digestion&#039;&#039;&#039;<br /> <br /> [[File:IMG 0858.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Subclone and Vector digestion&#039;&#039;&#039;<br /> <br /> [[File:IMG 0860.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Subclone of 931&#039;&#039;&#039;<br /> <br /> [[File:IMG 0859.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;PCR of 994_995&#039;&#039;&#039;<br /> <br /> [[File:IMG 0856.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;PCR of 3192-F1__3196&#039;&#039;&#039;<br /> <br /> [[File:IMG 0857.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;PCR of 3200&#039;&#039;&#039;<br /> <br /> [[File:IMG 0853.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;PCR of 3197&#039;&#039;&#039;<br /> <br /> [[File:IMG 0849.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;PCR of 998_1000&#039;&#039;&#039;<br /> <br /> [[File:IMG 0851.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;PCR of 3195_3199&#039;&#039;&#039;<br /> <br /> [[File:IMG 0852.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;3194 PCR&#039;&#039;&#039;<br /> <br /> [[File:IMG 0855.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;cDNA PCR of AH10_FE2&#039;&#039;&#039;<br /> <br /> [[File:IMG 0848.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 984_931&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Enzymes<br /> | Size<br /> | Note<br /> |-<br /> | 984<br /> | 2-5<br /> | Xba1+Nhe1<br /> | 957bp<br /> | &#039;&#039;&#039;#6,7,,8 seems good&#039;&#039;&#039;<br /> |-<br /> | 931<br /> | 1,6-8<br /> | HindIII+Xho1<br /> | 986bp<br /> | &#039;&#039;&#039;#5 seems good&#039;&#039;&#039;<br /> |}<br /> <br /> [[File:IMG 0871.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 2503_3122_3197_3191&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | lane<br /> | Enzymes<br /> | Size<br /> | Note<br /> |-<br /> | 2503<br /> | 1-4<br /> | Nde1+Xho1<br /> | 951bp<br /> | #1,2 seems good<br /> |-<br /> | 3122<br /> | 5-8<br /> | Nde1+Xho1<br /> | 1743bp<br /> | #5,6,8 seems good<br /> |-<br /> | 3197<br /> | 9-12<br /> | BamH1+Nco1<br /> | 1 band<br /> | may not good<br /> |-<br /> | 3191<br /> | 13-16<br /> | BamH1+Nco1<br /> | 722bp<br /> | #14,15,16 seems good<br /> |}<br /> <br /> [[File:IMG 0872.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 998_1000_994_995&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Enzymes<br /> | Size<br /> | Note<br /> |-<br /> | 998<br /> | 1-4<br /> | HindIII+Xho1<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 1000<br /> | 5-8<br /> | HIndIII+Xho1<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 994<br /> | 9-12<br /> | BamH1+Xho1<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 995<br /> | 13-16<br /> | BamH1+Xho1<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |}<br /> <br /> &#039;&#039;&#039;&#039;&#039;[[File:IMG 0873.JPG|RTENOTITLE]]&#039;&#039;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Miniprep of 3199&#039;&#039;&#039;<br /> <br /> [[File:IMG 0898.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Vector digestion&#039;&#039;&#039;<br /> <br /> [[File:Vector digestion (2).JPG]]<br /> [[Category:My ELN]]</div> Crm