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Yijia LI 7 2 2018 - Revision history
2026-10-18T08:47:53Z
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Crm at 22:24, 6 July 2018
2018-07-06T22:24:43Z
<p></p>
<p><b>New page</b></p><div>== Date:7/2/2018 ==<br />
<br />
'''1st PCR of 3218'''<br />
<br />
[[File:IMG 1054.JPG|RTENOTITLE]]<br />
<br />
'''Miniprep of 129_994_1000_3206'''<br />
<br />
{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Vector<br />
| Lane<br />
| Enzymes<br />
| Size<br />
| Clone#<br />
| Note<br />
|-<br />
| 129<br />
| 1801<br />
| 1-4<br />
| Nde1+HindIII<br />
| 2433<br />
| 2,4 look good<br />
| '''129-2;129-4'''<br />
|-<br />
| 994<br />
| 3225<br />
| 5-8<br />
| BamH1+Xho1<br />
| 609<br />
| 5,6,look good<br />
| Vecotor doesn't have BamH1 Site,'''994-1;994-2'''<br />
|-<br />
| 1000<br />
| 1708<br />
| 9-12<br />
| HIndIII+Xho1<br />
| <br/><br />
| 118<br />
| need to do PCR screening<br />
|-<br />
| 3206<br />
| 3165<br />
| 13-16<br />
| HIndIII+BamH1<br />
| 1460<br />
| <br/><br />
| need to do PCR screening<br />
|}<br />
<br />
[[File:IMG 1053.JPG|RTENOTITLE]]<br />
<br />
==Date:7/6/2018==<br />
<br />
[[File:IMG 1081.JPG]]<br />
[[Category:My ELN]]</div>
Crm