Crm at 18:44, 7 August 2018

2018-08-07T18:44:39Z

New page

== Date:7/30/2018 ==

'''129 vector digestion'''

[[File:IMG 1236.JPG|RTENOTITLE]]

'''998 PCR'''

[[File:IMG 12351.JPG|RTENOTITLE]]

'''3206 vector digestion'''

[[File:IMG 1230.JPG|RTENOTITLE]]

'''3244 vector digestion'''

[[File:IMG 1229.JPG|RTENOTITLE]]

'''3244 PCR'''

[[File:IMG 1225.JPG|RTENOTITLE]]

'''129 PCR'''

[[File:IMG 1227.JPG|RTENOTITLE]]

'''931-1 subclone'''

'''998 Vector1708 digestion'''

[[File:IMG 1239.JPG|RTENOTITLE]]

== Date:7/31/2018 ==

'''3244 PCR screening'''

[[File:IMG 1245.JPG|RTENOTITLE]]

'''3206 vector digestion'''

[[File:IMG 1247.JPG|RTENOTITLE]]

'''129 vector digestion'''

[[File:IMG 1248.JPG|RTENOTITLE]]

'''Miniprep digestion998_129_3244'''

[[File:IMG 1250.JPG|RTENOTITLE]]

'''Miniprep digestion 2_3244_129_998'''

[[File:IMG 1252.JPG|RTENOTITLE]]

== Date:8/2/2018 ==

'''vector digestion of 3206_998'''

[[File:IMG 1259.JPG|RTENOTITLE]]

= Individual report =

== 931 ==

'''Subclone of 931'''

Vector:1987pT7A3

Insert:

F1:1174 bp

F2:986 bp

[[File:Subclone931.JPG|RTENOTITLE]]

F1:Nde1+HindIII

F2:HindIII+Xho1

=== Date:5/29/2018 ===

'''Subclone of 931'''

'''Vector is 1987pT7A3'''

[[File:IMG 0859.JPG|RTENOTITLE]]

'''Miniprep digestion of 984_931'''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| #
| Lane
| Enzymes
| Size
| Note
|-
| 984
| 2-5
| Xba1+Nhe1
| 957bp
| '''#6,7,,8 seems good'''
|-
| 931
| 1,6-8
| HindIII+Xho1
| 986bp
| '''#5 seems good'''
|}

Digest with HindIII +Xho1 and Nde1+ HindIII, Nde1 + Xho1

using parental vector as control. Need to have marker

[[File:IMG 0871.JPG|RTENOTITLE]]

not good

3rd subclone
[[Category:My ELN]]

Yijia LI 7 30 2018 - Revision history

Crm at 18:44, 7 August 2018