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Yolanda Cloning - Revision history
2026-10-18T12:33:33Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=Yolanda_Cloning&diff=1512&oldid=prev
Hylei at 19:08, 12 August 2011
2011-08-12T19:08:58Z
<p></p>
<p><b>New page</b></p><div>8/4/2011 <br />
<br />
[[Image:Adx Lentivirus EcoRI BAMHI 2011 8 4.JPG]] <br />
<br />
<br> <br />
<br />
1: 1 kb ladder marker <br />
<br />
2: adx Lentivirus <br> <br />
<br />
<br> <br />
<br />
*1 uL adx Lentivirus <br />
*1 uL EcoRI (fast digestion) <br />
*1 uL BamHI (fast digestion) <br />
*1.5 uL 10x fast digestion buffer <br />
*10.5 uL water<br />
<br />
<br> <br />
<br />
====== ========= ======<br />
<br />
8/11/11 <br />
<br />
Digest with BamHI + XhoI <br />
<br />
*5 uL DNA<br />
*0.3 uL BamHI<br />
*0.3 uL XhoI<br />
*1.5 uL 10x buffer (fast digestion)<br />
*7.9 uL water<br />
<br />
Total: 15 uL<br />
<br />
[[Image:Multiple digestions2 IFP 2011 8 11.JPG]] <br />
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Lane 1: 1 kb ladder<br> <br />
<br />
Lane 2: IFP 1, pET 28b <br />
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Lane 3: IFP 3, pET 28b <br />
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Lane 4: Amp pAAV-IFP 254-1 <br />
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Lane 5: Amp pAAV-IFP 254-4 <br />
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<br> <br />
<br />
Notes: <br />
<br />
*IFP 3 pET 28b still shows good insert <br />
*Ran gel too long maybe<br />
<br />
<br> <br />
<br />
====== ============== ======<br />
<br />
8/12/11 <br />
<br />
Digest with BamHI + XhoI <br />
<br />
<u>Lanes 2-8 + 10-13</u>:<br />
<br />
*5 uL DNA <br />
*1.5 uL 10x buffer (fast digestion)<br />
*0.5 uL BamHI<br />
*0.5 uL XhoI<br />
*7.5 uL water<br />
<br />
'''Total''': 15 uL<br />
<br />
<u>Lane 14</u>:<br />
<br />
*10 uL DNA<br />
*3 uL 10x buffer (fast digestion)<br />
*1 uL BamHI<br />
*1 uL XhoI<br />
*15 uL water<br />
<br />
'''Total''': 30 uL<br />
<br />
[[Image:Multiple digestions3 2011 8 12.JPG]] <br />
<br />
<br> <br />
<br />
1: 1 kb ladder marker <br />
<br />
2: IFP-3 pET28b <br />
<br />
3: mNeptune-1 pET 286 <br />
<br />
4: mNeptune-2 pET 286 <br />
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5: mNeptune-3 pET 286 <br />
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6: sfGFP2 pET 286 <br />
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7: Taq RFP-1 <br />
<br />
8: Taq RFP-2 <br />
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9: 1 kb ladder marker <br />
<br />
10: EGFP <br />
<br />
11: Amp pAAV-IFP 2 (Mindy) <br />
<br />
12: Amp pAAV-IFP 3 (Mindy) <br />
<br />
13: Amp pAAV-IFP 5 (Mindy) <br />
<br />
14: pAAV-CFP 4<br><br />
<br />
<br />
<br />
Notes: <br />
<br />
[[Category:Cloning]]</div>
Hylei