Editing 2773 (section)

= Cloning =

== PCR using Taq ==

[[File:2763 2773 PCR.JPG|RTENOTITLE]]

[[File:2773 XhoI.JPG|RTENOTITLE]]

[[File:2759 2763 2773 XhoI.JPG|RTENOTITLE]]

[[File:2760 2763 2770 2773 PCR.JPG|RTENOTITLE]]

== PCR using Thermofisher SuperFi II ==

=== PCR ===

PCR from genomic DNA using superhifi II enzyme mix

6/6/2020 [[File:2763 2772 2773 2779 PCR.JPG|RTENOTITLE]]

=== Miniprep Digestion ===

[[File:2773 2779 digestion.JPG|RTENOTITLE]]

[[File:2773 XhoI NdeI.JPG|RTENOTITLE]]

[[File:06092020 2759 2773 gel.JPG|RTENOTITLE]]&nbsp;06092020 [[File:2763 2773 pMiniT2 NdeI XhoI.JPG|RTENOTITLE]]

6/10/2020

digestion miniprep DNA with Xho1

[[File:2773 2763 digestion.JPG|RTENOTITLE]]

2773 in pJet1.2

digested with Xba1-Xho1

2696 Nco1-Xho1 digested vector is for 2772

[[File:2773 pJet.JPG|RTENOTITLE]] 20200616 2773 pJet NdeI+XhoI in Cutsmart, 37oC, 1hour [[File:2746 2763 2773 2779 pJet digestion.JPG|RTENOTITLE]]

== 2773 gibson ==

[[File:2773 gibson insert pcr.JPG|RTENOTITLE]]

 [[File:2773 gibson vector.JPG|RTENOTITLE]]

[[File:2773 gibson vector R.JPG|RTENOTITLE]]

[[File:2773 gibson insert pcr R.JPG|RTENOTITLE]]
[[Category:Cloning]]