Editing BradFord Assay (section)

== Incompatible Substances /Amount Compatible ==

The concentration listed below is the maximum amount of material which can be present in the&nbsp;protein sample without causing interference in the standard protocol when 20 ul protein sample is&nbsp;used for Bradford assay.

<br/><br/>'''Buffer Systems'''<br/>ACES, pH 7.8 100 mM<br/>N-Acetylglucosamine in PBS, pH 7.2, 100 mM<br/>Bicine, pH 8.4 100 mM<br/>Bis-Tris, pH 6.5 100 mM<br/>Calcium chloride in TBS, pH 7.2 10 mM<br/>CelLytic B Reagent undiluted, no interference<br/>CHES, pH 9.0 100 mM<br/>Cobalt chloride in TBS, pH 7.2 10 mM<br/>EPPS, pH 8.0 100 mM<br/>Ferric chloride in TBS, pH 7.2 10 mM<br/>Glycine 100 mM<br/>HEPES, pH 7.5 100 mM<br/>Imidazole, pH 7.0 200 mM<br/>MES (0.1 M), NaCl (0.9%), pH 4.7 undiluted<br/>MES, pH 6.1 100 mM<br/>MOPS, pH 7.2 100 mM<br/>Nickel chloride in TBS, pH 7.2 10 mM<br/>PBS; Phosphate (0.1 M), NaCl (0.15 M), pH 7.2, undiluted<br/>PIPES, pH 6.8 100 mM<br/>Sodium acetate, pH 4.8 180 mM<br/>Sodium bicarbonate 0.1 M<br/>Sodium citrate, pH 4.8 or pH 6.4 200 mM<br/>Sodium Citrate (0.6 M), MOPS (0.1 M), pH 7.5, undiluted<br/>Sodium phosphate 0.1 M<br/>TBS; Tris (25 mM), NaCl (0.15 M), pH 7.6, undiluted<br/>Tricine, pH 8.0 100 mM<br/>Triethanolamine, pH 7.8 100 mM<br/>Tris 2.0 M<br/>Tris (25 mM), Glycine (192 mM), pH 8.0, undiluted<br/>Tris (25 mM), Glycine (192 mM), SDS (0.1%), pH 8.3, 1:2 dilution<br/>Zinc chloride in TBS, pH 7.2 10 mM<br/>Buffer Additives<br/>Ammonium sulfate 1.0 M<br/>Aprotinin 10 mg/L<br/>Asparagine 10 mM<br/>Cesium bicarbonate 0.1 M<br/>Glucose 1.0 M<br/>Glycerol 10%<br/>Guanidine•HCl 3.5 M<br/>Hydrochloric Acid 0.1 M<br/>Imidazole, pH 7.0 200 mM<br/>Leupeptin 10 mg/L<br/>Phenol Red 0.5 mg/ml<br/>PMSF 1 mM<br/>Sodium azide 0.5%<br/>Sodium chloride 5.0 M<br/>Sodium Hydroxide 0.1 M&nbsp;<br/>Beyotime Institute of Biotechnology<br/>Sodium orthovanadate in PBS, 1 mM<br/>Thimerosal 0.01%<br/>Sucrose 10%<br/>TLCK 0.1 mg/L<br/>TPCK 0.1 mg/L<br/>Urea 3.0 M 3.0 M<br/>Detergents<br/>Brij®-35 0.125%<br/>Brij®-52 0.031%<br/>CHAPS 5%<br/>CHAPSO 5%<br/>Deoxycholic acid 0.050%<br/>Nonidet P-40 (Igepal CA-630) 0.5%<br/>N-Tetradecyl-N 0.125%<br/>Octyl ®-glucoside 0.5%<br/>Octyl ®-thioglucopyranoside 3%<br/>SDS 0.125%<br/>Span® 20 0.5%<br/>Triton® X-100 0.125%<br/>Triton® X-114 0.125%<br/>Triton® X-305 0.5%<br/>Triton® X-405 0.5%<br/>Tween® 20 0.062%<br/>Tween® 60 0.1%<br/>Tween® 80 0.062%<br/>Chelating agents<br/>EDTA 100 mM 100 mM<br/>EGTA 2 mM 2 mM<br/>Sodium citrate, pH 4.8 or pH 6.4 200 mM<br/>Reducing & Thiol Containing Agents<br/>2-Mercaptoethanol 1.0 M<br/>Ascorbic acid 50 mM<br/>Cysteine 10 mM<br/>Dithioerythritol (DTE) 1 mM<br/>Dithiothreitol (DTT) 5 mM<br/>Potassium thiocyanate 3.0 M<br/>Solvents<br/>Acetone 10%<br/>Acetonitrile 10%<br/>DMF 10%<br/>DMSO 10%<br/>Ethanol 10%<br/>Methanol 10%<br/>Note: This is not a complete compatibility chart. There are many substances that can affect&nbsp;different proteins in different ways. One may assay the protein of interest in deionized water alone,&nbsp;then in buffer with possible interfering substances. Comparison of the readings will indicate if an&nbsp;interference exists.<br/>Note: Reagents that change the pH of the assay or contains high levels of detergents will interfere&nbsp;with the Bradford assay.