Editing LCC 500-501 Operation (section)

== Operation <br>Start-up <br>  ==

1. Check that all components are correctly installed 

2. Turn on the pumps, monitors, recorder and fraction collector. 

3. Set the flow rate on the pumps to zero and press the RUN button (E-50, P-500, P-6000). 

4. Set the three chart speed selection buttons on the recorder to 10, mm/sec and "ext". Set the input range selection buttons for the channel connected to GRADIENT 1 V on the LCC-501 Plus to 1 V (10x100 mV) and the buttons for die channel connected to the monitor to the appropriate voltage. Press the input range selection button 0 on both channels to adjust both pens to zero with the zero control knob. Release both input range selection buttons 0 (REC-102). 

5. Set the appropriate sensitivity with the sensitivity range selector on the UV monitor and adjust the baseline with the baseline knob or press "AUTO ZERO" on the UV-M II. 

6. Turn On the LCC-501 Plus. 

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7. Enter the Calibration block and check the kalor for method base and calibration values for the pumps, recorder etc. 

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8. Program the fraction collector and set it in the start position. 

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9. Check that the pumps are filled with the correct buffers by activating the wash program in the Manual block. 

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10. To get started as fast as possible there are 9 different preprogrammed methods within the sminvare of the LCC-501 Plus. They am easily copied into Method banks in methods 0~8 with the command a CLEAR MEMORY 501. 

The methods are for specific columns sizes and techniques, to optimise your methods follow the instructions included with the columns. 

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Note The preprogrammed methods wiil not write over existing methods. 

11. If the method is to be programmed before starting the run, it is convenient to equilibrate the column during this period by setting the initial conditions through the Manual block. 

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12. Program the method. 

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13. Check the method in the Review block or by listing it on the printer. 

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14. Run the method. <br>