Editing Phage Display Library Screening (section)

== Library selection on immunotubes ==

All selections were performed using recombinant or commercially available&nbsp;antigens with purity >90%.

<br/>Coat Immunotubes (Nunc; Wiesbaden, Germany)&nbsp; with antigen at a&nbsp;concentration of 50 μg/ml in PBS over-night at room temperature. 

Rinse&nbsp; Immunotubes with PBS

Block with 2% MPBS for 2 hours at room temperature .

Rinse with PBS

Add >10<sup>12</sup> phage particles in 2% MPBS&nbsp; tothe immunotubes. 

Incubate the immunotubes&nbsp; on a shaker for 30&nbsp;minutes and then for 90 minutes standing upright at room temperature. 

Wash unbound&nbsp;phage away by rinsing the immunotubes ten times with PBS 0.1%&nbsp;Tween 20 and ten times with PBS. 

Elute the bound phages&nbsp; by&nbsp;incubation of 1 ml 100 mM triethylamine for 5 minutes. 

Add 0.5 ml 1 M Tris-HCl pH 7.4. to neutralize triethylamine