Editing Recover small DNA fragment from Gel (section)

== Ethanol Precipitation Protocol ==

1.&nbsp; Run&nbsp;&nbsp;small PCR DNA fragments obtained&nbsp; &nbsp;on agarose gels.

2. Cut out DNA fragments and place in a sterile 1.5 mL microcentrifuge tube.<br/>3.&nbsp; Ground gel with&nbsp; a metal rod and&nbsp; then add &nbsp;150 μL of TE buffer&nbsp;<br/>4.&nbsp;Frozen&nbsp; gel in tube using dry ice&nbsp;&nbsp;<br/>5.&nbsp;Incubate&nbsp; the tube at 72 °C for 3 min.<br/>6.&nbsp;Immediately following incubation, spin the tubes&nbsp; at 10,000 x g for 30 s at room temperature (RT).<br/>7. Transfer the solubilized gel solution to&nbsp;a new sterile tube (1.5 mL)<br/>8.&nbsp; Add&nbsp;&nbsp;1/10 vol of sodium acetate (3 M, pH 5.2) and 2.5 vol of cold ethanol (or isopropyl alcohol).<br/>9. Incubate the tubes&nbsp; overnight at −20 °C. Note: The incubation time for the precipitation depends of the initial concentration of DNA in the sample.<br/>10.&nbsp;Spin at 16,000 x g for 20 min at RT<br/>11.Wash&nbsp;with 600 μL of 70% ethanol (v/v)

12. Air dry&nbsp;and re-suspend&nbsp;by adding TE buffer or water to the desired volume.<br/>13.&nbsp;Store&nbsp; at −20 °C until further use.