Editing Synthesis SS-PBAA-PEI (section)

=== SS-PBAA-PEIs<br>  ===

1.&nbsp; Add 2-amine-1-ethanol (EA) or 5-amino-1-pentanol (PA) to CBA. 

Sigma: 

2-amino-1-ethanol (EA):&nbsp;494577-1G, $133.50 

5-amino-1-pentanol (PA): 123048-10G, $33.80; 123048-50G, $121.50<br><br> 

2. 4 different&nbsp; SS-PBAA1-PEIs can be synthesized by varying the stoichiometric ratio (r) of the monomers (i.e. rEA/CBA or rPA/CBA)<br> 

3. For example: <br> 

Stir a mixture of EA (0.109 g, 1.8 mmol) and CBA (0.520 g, 2 mmol, stoichiometric ratio rEA/CBA = 0.9) in 5 ml of 25% aqueous methanol in the dark at 60 °C under a nitrogen atmosphere for 48 hours. This will yield&nbsp; a homogeneous solution. <br> 

Add 0.276 g PEI in 2 ml of 25% aqueous methanol&nbsp; to the SS-PBAA1 solution, react 24 hours at 45 oC under a nitrogen atmosphere. This will yield SS-PBAA1-PEI (4:1).<br> 

4. Dilute the transparent solution with water to 30 ml and acidify with 6 M HCl to pH 4, then dialyze against distilled water (molecular weight cut-off 8000) for 2 days to remove the unreacted reactants. <br> 

5. Lyophilize for 2 days to obtain SS-PBAA1-PEI. <br> 

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[[Category:Transfection]] [[Category:Paul]]