Editing Synthesis of PHP-Cho (section)

== Synthesis of PEI-Cho ==

'''9/22/14'''

300 mg PEI18 + 1 ml DCM + 10 ul trethtlamine (TEA)

100 mg CHol-Chl in 0.5 ml DCM (0.2 mg/ul)

{| width="492" cellspacing="1" cellpadding="1" border="1"
|-
| Sample #<br/>
| PEI18 mg<br/>
| PEI18 ul<br/>
| Chol-Chl mg<br/>
| CHol-Chl, ul<br/>
| DCM<br/>
| After reaction<br/>
|-
| E1<br/>
| 30 mg<br/>
| 100 ul<br/>
| 10 mg<br/>
| 50 ul<br/>
| 0<br/>
| Not soluble in H2O<br/>
|-
| E2<br/>
| 30 mg&nbsp;&nbsp;<br/>
| 100 ul<br/>
| 7.5<br/>
| 37.5<br/>
| 12.5<br/>
| Not soluble in H2O<br/>
|-
| E3<br/>
| 30 mg
| 100 ul
| 5<br/>
| 25<br/>
| 25<br/>
| Not soluble in H2O<br/>
|-
| E4<br/>
| 30 mg
| 100 ul
| 2.5<br/>
| 12.5<br/>
| 37.5<br/>
| Partial soluble in H2O<br/>
|-
| E5<br/>
| 30 mg
| 100 ul
| 1<br/>
| 5<br/>
| 45<br/>
| soluble in H2O<br/>
|-
| E6<br/>
| 30 mg
| 100 ul
| 0<br/>
| 0<br/>
| 50<br/>
| soluble in H2O<br/>
|}



React at 4~8 oC (cold room) o/n

+ 100 ul H2O, + 200 ul Ethyl acetate to extract, spin, remove top organic phase, + 10 ul H2O to bottom phase, repeat washing with EtoAc several time, dry in the fume hood

--> total vol ~=100 ul, 0.3 mg/ul