Editing Total-RNA-From-Rabbit-Spleen (section)

= '''Materials''' =

1. Plasticware: 50-ml conical tubes (e.g., Falcon tubes; BD Biosciences, San Jose, CA); RNase-free 50-ml centrifuge tubes (e.g., Nalgene 3139 Oak Ridge centrifuge tubes; Thermo Fisher Scientific, Waltham, MA); RNase-free 1.5-ml microcentrifuge tubes (e.g., Biopur tubes; Eppendorf, Westbury, NY);

RNase-free filter pipette tips (e.g., ART; Molecular BioProducts, San Diego, CA).

2. Spleen and bone marrow from one femur of an immunized rabbit, each in 10 ml TRI reagent (Molecular Research Center,<br>Cincinnati, OH; store at 4 oC) in 50-ml conical tubes stored on dry ice or at -80 oC (see Note 1).

3. BCP: 1-bromo-3-chloro-propane (Molecular Research Center). Store at room temperature.

4. Isopropanol (Sigma-Aldrich, St. Louis, MO). Store at room temperature.

5. Freshly prepare 70% (v/v) ethanol (Sigma-Aldrich) in RNasefree water (Ambion, Austin, TX) and keep at roomtemperature.

6. RNA storage solution: RNase-free 1 mM sodium citrate (pH 6.4) (Ambion). Store at -20oC.

7. RNase-free 3 M sodium acetate (pH 5.2) (Ambion). Store at room temperature.