Editing NGS Library Preparation (section)

==== Sequencing. ====

Multiplex libraries were prepared using barcoded primers and a median insert size of 150 bp (increased to 200 bp with the modified method). The pooled libraries from 3 initial Norovirus and 2 HCV samples were sequenced on an Illumina MiSeq with 150 bp paired end reads following standard Illumina protocols. An average of 0.5 Gb of sequence was produced per sample. A larger pool of 77 Norovirus samples was sequenced on an Illumina HiSeq 2000 with 100 bp paired end reads following standard Illumina protocols.