Editing LCC 500-501 Operation (section)

===== To wash the column:  =====

Connect a column to port 1 of the switching valves. Be sure that there is liquid in the tube directly connecting to the column so that no air enters the column. If the tube is dry then switch the pumps on to run to the column before connecting the column to get liquid in the tube. Let fluid run into any connecting nozzles to remove any air. 

Connect the bottom of the column to the UV monitor tubing, again assuring not to force any air into the detector's flow cell. If you do end up with air in the flow cell, this may present as the detector signal jumping all over the place and the plotter flying up and down wildly. If this happens, use a syringe to force fluid through the flow cell to try and get the bubble out. 

Set the ml/min to something low, like 0.5 ml/min and the chart recorder to 1 or 0.5 cm/ml 

Watch the UV and conductivity meters and allow them to equilibrate, meaning that the conductivity monitor and detector signals stabilize. 

Watch that the pressure does not go above 2 MPa with any size exclusion column, nor above 0.5 MPa with any ion exchange column. 

You can increase the ml/min if the pressure remains low.