Editing LCC 500-501 Operation (section)

===== Trouble with columns:  =====

If the back pressure on the column is high, there can be several problems. First, when ethanol is present in the column, the pressure will be higher than usual. This is fine, just use a low flow rate until the ethanol is flushed out. If the pressure remains high, the first place to check is the pre-column filter. Replace the filter with an extra from the FPLC drawer. If this does not help, then the flow cell my be clogged or the column may be dirty. Check column or UVis information sheets to determine cleaning solutions. 

If the alarm is going off on the column for any reason, the machine will usually pause itself. Push the alarm reset button on the main computer to reset, then push continue (if you have figured out what caused the alarm and corrected it).