Editing LCC 500-501 Operation (section)

===== Loading a sample:  =====

Connect a sample loop of similar size to the volume you wish to load on the column to ports 2 and 6 on the switching valve. Use a syringe with water in it to wash the sample loop by inserting the syringe into the syringe port on the switching valve. Push the syringe in all the way, but do not push it too hard. Inject the water through the sample loop, it will come out of a tube which should be held above the level of the loading syring so that the water does not drain out when done. Again, this is to keep air bubbles from forming in the sample loop which then might be injected onto a column. 

Then load sample in syringe into sample loop. The sample volume should ideally be larger than the sample loop so that no air bubles will be present in the loop after loading. If the sample volume is smaller than the loop be careful that the water injected for cleaning is straight against the sample volume (no air bubble). 

Leave the syringe in place after loading the sample loop. (leave syring in place during all times that the valve is switched to 1.2) 

When the valve is switched to 1.2, the sample loop will be part of the flow to the column and the sample will inject onto the column. Once enough volume has gone through the loop to clear it out, switch the valve back to 1.1. Now you can safely remove the syringe and clean the loop (or reload the sample loop).