Editing LCC 500-501 Operation (section)

===== Running a method:  =====

The gel filtration column is run with a constant buffer percentage and does not require a formal method to run. This can be run directly off the manual control and the fraction collector can be run manually as well (see info. about fraction collector below). However if you wish to write a method for it you may. 

The ion exchange columns require a salt gradient for elution. This requires a method file. Method files are stored in 5 different method banks (1-5) and then in 10 different methods in each bank (0-9). 

To set up a method: 

There are some standard methods that can be loaded into the method bank 5 with the command Clear Memory 501. See inside the back cover of the controller manual for the standard methods. 

To edit a method, choose “method file” from the front panel. Then type 5 and enter for the 5th bank. Then use the forward button to get to “program method” and enter the method you wish to edit (example 0). Push “forward” to step through the commands of the method. An example with a few notes is below. The commands such as ml/mark and integrate have to do with the printing out of a small version of the run on the little onboard printer as the method runs. 

Use the delete key to remove a command. Use the insert key to insert a command; once you have pushed the insert key you can scroll through the commands with the forward button until you reach the one you wish to insert, then hit enter. At this point you can enter the time you want the command for. Then use the change key to change the value of the newly inserted command. Use the change key to change the time or value of a specific command. 

When you have the method the way you want it, choose exit from the front panel. 

Now you can run your method by typing the method number and hitting enter (it will run the method of the method bank you were last in) 

example of method: 

{|
|-
| 0.00 
| conc&nbsp;%B 
| 0.0
|-
| 0.00 
| ml/min 
| 1.00
|-
| 5.00 
| valve.pos 
| 1.2 
| this is to '''Inject''' sample loop volume
|-
| 5.00 
| port.set 
| 6.1 
| this turns the '''fraction collector''' to run
|-
| 5.00 
| clear data
|-
| 5.00 
| monitor 
| 1
|-
| 5.00 
| level&nbsp;% 
| 5.0
|-
| 5.00 
| min/mark 
| 2.0
|-
| 5.00 
| integrate 
| 1
|-
| 10.0 
| conc&nbsp;%B 
| 0.0
|-
| 10.0 
| valve.pos 
| 1.1 
| '''Load'''; this stops going through th sample loop
|-
| 30.00 
| conc&nbsp;%B 
| 35.0
|-
| 34.00 
| conc&nbsp;%B 
| 100
|-
| 38.00 
| conc&nbsp;%B 
| 100
|-
| 38.00 
| port.set 
| 6.0 
| this stops the fraction collector
|-
| 38.00 
| integrate 
| 0
|-
| 38.00 
| prt pk 
| 1.05
|-
| 38.00 
| conc&nbsp;%B 
| 0.0
|-
| 43.00 
| conc&nbsp;%B 
| 0.0
|}

To switch between the values being given in min or in ml, use the calibration key and in the first choice use 0 for minutes, 1 for ml, and 2 for dl. Then use the exit key to leave the calibration mode.