Editing TREC ASSAY (section)

== Materials ==
<div>
{|
|-
| Name
| Company
| Catalog Number
| Comments
|-
| Histopaque-1077
| Sigma Aldrich SRL
| 10771-500 ML
| density gradient separation method
|-
| QIAamp DNA Blood Mini Kit (250)
| QIAGEN
| 51106
| DNA extraction
|-
| Unmodified DNA Oligonucleotides HPSF 0.01 mmol
| Eurofins MWG Operon/Carlo Erba Reagents S.r.l&nbsp;
| Resuspend the lyophilized product to 100 pmol/µl
|-
| AmpliTaq DNA Polymerase: including 10x Buffer II and 25 mM MgCl2
| Applied Biosystems/Life-Technologies
| N8080156
|-
| GeneAmp dNTP Blend (100 mM)
| Applied Biosystems/Life-Technologies
| N8080261
|-
| TOPO TA Cloning Kit for Subcloning
| Invitrogen/Life-Technologies
| K4500-01
|-
| XL1-Blue Subcloning Grade Competent Cells
| Stratagene
| 200130
|-
| PureYield Plasmid Miniprep System
| Promega
| A1223
|-
| SpeI 500U
| New England Biolabs
| R0133S
|-
| HindIII-HF 10,000 U
| New England Biolabs
| R3104S
|-
| PureYield Plasmid Midiprep System
| Promega
| A2492
|-
| XhoI 5,000 U
| New England Biolabs
| R0146S
|-
| TRIS Utrapure
| Sigma Aldrich SRL
| T1503
|-
| EDTA
| Sigma Aldrich SRL
| E5134
|-
| TE buffer (1 mM TRIS and 0.1 mM EDTA)
|-
| TaqMan Universal PCR Master Mix
| Applied Biosystems/Life-Technologies
| 4364338
|-
| Dual labeled probes HPLC 0.01 mmol
| Eurofins MWG Operon/Carlo Erba Reagents S.r.l&nbsp;
| Resuspend the lyophilized product to 100 pmol/µl
|-
| NanoDrop 2000c spectrophotometer
| ThermoFisher
|-
| Applied Biosystems 2720 Thermal Cycler
| Applied Biosystems/Life-Technologies
| 4359659
|-
| Fast 7500 Real-Time PCR system
| Applied Biosystems/Life-Technologies
|-
| SDS Sequence Detection Software 1.4
| Applied Biosystems/Life-Technologies
|}
</div>