Editing CHO Cell Culture (section)

== <br>Prepare supplements  ==

–– HT: Add 10 mL of EX-CELL CD CHO Fusion to each vial of HT (2 vials needed for 1L of growth media).<br>This creates a 50X solution. Discard any unused resuspended HT. 

–– Folinic Acid: Resuspend folinic acid in sterile water to create a 1 mM stock solution. Sterile filter. Make 1 mL aliquots and store at -20 °C. Thaw prior to use. 

–– Glutamine: Thaw glutamine in 37 °C water bath until completely dissolved. After thawing, glutamine may be stored at 4 °C for up to 2 weeks.<br>Add media supplements to EX-CELL CD CHO Fusion to achieve the final concentrations listed in the table above.<br>* Filter sterilize the complete growth media.<br>* Store growth media at 4 ̊C when not in use. Discard any unused media after 1 month.