Editing Enterokinase Purification (section)

== Buffers<br> ==

'''Lysis Buffer'''<br>

Tris 50 mm, 3.03 g (for 500 mL), pH 7.<br>NaCl 500 mM<br>EDTA 20 mm<br>Triton X-100 6%<br><br>

'''Suspension Buffer '''<br>Tris 50 mM 3.03 g (for 500 mL), pH 7.0<br>EDTA 20 mM 2.92 g<br>



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'''Solubilization Buffer I'''

Guanidine-HCl 7.5 M, 716.48 g L−1<br>Tris 50 mM, 6.06 g L−1<br>β-Mercaptoethanol 100 mM, 6.97 mL L−1<br>pH (native) ≈9





<br>'''Citrate Buffer'''<br>Citrate 5 mM 4.20 g (for 4 L)<br>pH 3.5–4.0<br><br>

'''Solubilization Buffer II'''

Guanidine-HCl 7.5 M, 358.24 g (for 500 mL)<br>Tris 50 mM 3.03 g<br>pH 4.0–4.5



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'''Refolding Buffer'''<br>Arginine 0.5 M 348.4 g (for 4 L)<br>Tris 50 mM 24.24 g<br>CaCl2 20 mM 11.80 g<br>EDTA 1 mM 1.16 g<br>Cysteine-HCl 5 mM 3.52 g<br>Cystine 0.5 mM 0.48 g<br>pH 8.3 at RT







Note: Dissolve cystine in 5 mL 2 N HCl<br><br>

<br> '''Storage Buffer'''<br>Tris 50 mM 24.24 g (for 4 L)<br>NaCl 50 mM 11.68 g<br>pH 7.5<br>'''<br>High-NaCl Buffer'''<br>Tris 50 mM, 3.03 g (for 100 mL)<br>NaCl 5M, 29.22 g<br>pH 7.5



<br>'''Binding Buffer'''<br>Tris 50 mM 3.03 g (for 500 mL)<br>NaCl 500 mM 14.6 g<br>pH 7.5

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'''Elution Buffer 100.'''<br>Binding Buffer 100 mM 50 mL<br>Benzamidine-HCl 0.78 g<br>pH 7.5



<br>'''Cryobuffer'''<br>Tris 50 mm 3.03 g (for 500 mL)<br>NaCl 50 mm,&nbsp; 1.46 g<br>Glycerol 10 % (v/v), 50 mL<br>pH 7.5<br><br><br>