Editing Hybridoma production (section)

=== Immunization ===

<br/>1. Use BALB/c female mice, 8-10 weeks of age, no preferred vendor

2. Immunize mouse three times (d0, d14, d21) i.p. with protein of interest and 100µlGerbu adjuvant (total volume of 200µl). Use 1-100ug of protein - depending on&nbsp;purity, concentration, and availability. The protein amount for the first&nbsp;immunization is twice the amount of all subsequent immunizations.

3. Bleed mouse before each immunization for serum collection (~100ul).

4. Perform mouse serum ELISA after d21 sampling. The titer from day 0 to day 21 should increase by 100-1000 fold.

5. When the titer is sufficient (usually after three immunizations, occasionally more), boost with protein (no adjuvant, add 100µl 1xPBS instead) three times&nbsp;three days in a row (d28, d29, d30)&nbsp;

6. Perform fusion on d31