Editing LCC 500-501 Operation (section)

== Terminology<br>  ==

===  ===

=== CALIBRATE PUMP A <br><br>  ===

Allows calibration of a pump connected to the connector PUMP A. For calibrating a peristaltic pump, connect it to the PUMP A socket and follow the procedure below. Do not forget to change the connections and re-enter PUMP A calibration value after you have finished the procedure. The<br>procedure is according to the following scheme:<br>1. Fill the pump with suitable liquid, e.g. water, (before connecting it to LCC-501 plus).<br>2. Connect the pump to the PUMP A connection on rear panel. Turn on the pump.<br>3. Set a representative flow rate on the control panel of the pump, (approx. 1 ml/min. ). Place the outlet from the pump into a graduated cylinder. <br>4. Press [do store]. The run LED is switched on and pump A control from the LCC-501 Plus is released so pump A can start with the flow rate set on the pump. The LCC-501 Plus is now counting pulses from the stepper motor in the pump.<br>5. Press [end] when a volume large enough to be measured with good accuracy has been delivered, e.g. 10 ml.<br>6. VOLUME.ML is displayed (or VOLUME. DL if dl mode is chosen).<br>Enter the collected volume and press [do store].<br>7. The calibration mode is terminated and the display will show PUMP CAL AB as above but with a new calibration value.<br><br> 

The calibration value for the Pump P-50 is 234 pulses/0.1 ml, for the P-500 is 110 pulses/0.1 ml <br><br> 

'''P-1ポンプのキャリブレーション方法'''<br>ポンプのキャリブレーション方法<br>1 P-1 ポンプをコミュニケーションケーブルでコントロール背面の Pump A のインターフェイスに接続<br>2 P-1 ポンプの流速を、5、×10 にセット<br>3 P-1 ポンプ出口に 10 ml 程度のメスシリンダーを置き、CALIBRATE PUMP Aで&nbsp; <u>do/store </u>ボタン<br>4 メスシリンダーに 5 ml 程度たまったら、end ボタン<br>5 ディスプレイに Volume ml と表示されるので、測定した溶液の Volume 値 をコントローラのタッチパネルで入力(検量値(1ml 送液するのに必要なパ ルス数)は自動的に PUMP CALABに入力される)<br>6 ポンプCとしてのみ、P-1 ポンプを使用する場合には、 PUMP CALABから 一つ進めて PUMP CAL Cに同じ値を入力し、かつ、PUMP CALABには 使用するポンプの検量値を入れ直す。<br>8 さらに、1でコントロール背面の Pump Aのインターフェイスに接続した ケーブルを、Pump C のインターフェイスに接続し直す 

<br>'''P1 tubings'''<br> 

Silicone tubing/P-1 i.d. 1.0 mm 2 19-4692-01<br>Silicone tubing/P-1 i.d. 2.1 mm 2 19-4691-01<br>Silicone tubing/P-1 i.d. 3.1 mm 2 19-4690-01<br>Fluoro-rubber tubing/P-1 i.d. 1.0 mm 2 19-7626-01<br>Fluoro-rubber tubing/P-1 i.d. 2.1 mm 2 19-7627-01<br>Fluoro-rubber tubing/P-1 i.d. 3.1 mm 2 19-7628-01<br>PVC tubing/P-1 i.d. 1.0 mm 2 19-7629-01<br>PVC tubing/P-1 i.d. 2.1 mm 2 19-7630-01<br>PVC tubing/P-1 i.d. 3.1 mm 2 19-7631-01<br> 

=== PT<br>  ===

<br>Shows the status of attached outports i.e. active or inactive e.g. .<br>0.0.1.0.0.0.0.0.0.0 indicates that port 2 is active. To set port 5 active enter 5.1.<br>Port 0 is switch valve or user-definable outport<br>Ports 1-4 are user-definable outports<br>Port 5 is fraction collector pause (1), continue (0)<br>Port 6 is fraction collector run (1), end (0)<br>Port 7 is fraction collector recycle (1), reset (0), recycle is for Frac-200.<br>Ports 8-9 are user-definable outports.<br> 

<br> 

=== WASH A.B.<br><br>  ===

Activates the wash program in pumps A and B if they have the function (e.g. P-500 and P-6000). To activate the wash program in pump A enter 1.0; to activate the wash program in pump B enter 0.1; to activate the wash program in both pumps, A and B, enter 1.1.<br> 

=== <br><br>SYNC A.B <br><br>  ===

Drives the pump pistons to an end position if the pumps are provided with the function (e.g. P-500 and P-6000). Useful for gradients of very small volumes. Enter 1.0 for driving the pump A piston to an end position, 0.1 for pump B and 1.1 for both pumps.<br><br> 

=== PORT.SET <br><br>  ===

Sets outports active or inactive e.g. 3.1 sets port number 3 active.<br>1 = active; 0 = inactive.<br>Note: Default means active is electrically 0 V on ports 0 and 3-9 and floating voltage on ports 1 and 2. <br> Port 0 (when not used for switch valve) rear panel no. 6<br> 1 rear panel no. 18<br>2 rear panel no. 19<br>3 rear panel no. 2<br>4 rear panel no. 4<br>5 fraction collector, pause (1), continue (0)<br>6 fraction collector, run (1), end (0)<br>7 fraction collector, recycle (1), reset (0)<br>8 rear panel no. 21<br>9 rear panel no. 22<br><br> 

These instructions were originally written by Katarina Midelfort in 1999. Please get trained by current Wittrup lab personnel before using for the first time. Some things may have changed. 

The fast protein liquid chromatography (FPLC) instrument is from Pharmacia. It is comprised of pumps, switching valves, sample loop, separation column, UV detector, conductivity meter, sample collector, and plotter. The instrument is run from an internal computer. 

'''FLOW C''': Pump C flow rate<br>'''VALVE 0.0''': VALVE 1.3<br><br>