Editing M9 (section)

=== Method  ===

To prepare 1L of media, add:<br> 

*200mL 5X M9 salts solution 
*to ~800mL of distilled water 
*Add 15g of agar media if agar plates are to be poured.

After autoclaving, swirl to mix evenly and "temper" at room temperature until you can place your hand on the flask for 2 second (an adult should do this!), then add the following: 

*2mL of 1M MgSO4 solution 
*0.1mL of 1M CaCl2 solution 
*20mL of 20% glucose

Swirl to mix evenly. 

Liquid media should be distributed to sterile bottles or tubes with a pipette and pipettor. Small bottles can hold 250mL of media for use when needed, for instance, or aliquots of 5mL or 10mL can be transferred to sterile, capped test tubes. 

If plates are to be poured, ~25mL of media should be added per Petrie dish and allowed to solidify at room temperature. Poured/solid plates can be stacked and replaced in the original plastic sleeve in which they came. Label the sleeve with the type of media and date poured. <br> 

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