Editing Minimal Medium (section)

== Recipe 2<br>  ==

1x M9 salts<br> 2mM MgSO4<br> 0.1mM CaCl2<br> 0.4% carbon source (e.g. glycerol, glucose, etc.)<br> In sterile H2O <br> 

'''For 1L of media''' 

500ml 2xM9 salts ∗<br> 20ml 0.1M MgSO4 ∗<br> 200μl 0.5M CaCl2 ∗<br> 469.8ml sterile deionized H2O ∗ (if carbon source to be added)<br> 479.8ml sterile deionized H2O ∗ (if no carbon source to be added, useful as a control for sugar metabolism) 

Combine above solutions using sterile technique. (May notice some precipitation during preparation but precipitate should go back into solution once volume is brought up to 1l with sterile H2O.)<br>Add antibiotic as appropriate and store at 4°C <br> 

'''5X M9 salts contains:''' 

64 g Na2HPO4.7H2O<br> 15 g KH2PO4<br> 2.5 g NaCl<br> 5 g NH4Cl 

dissolved in deionized water to a final volume of 1 L <br> 

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'''M9-glu''' contains glucose: In the basic recipe take 800 ml sterile water and supplement with 100 ml 20% (w:v) sterile filtered glucose before adding M9 salts, magnesium and calcium salts. [This and following instructions refer to the basic M9 medium recipe.] 

'''M9-CA '''contains casamino acids: In the basic recipe take 800 ml sterile water and supplement with 100 ml 10% (w:v) autoclaved casamino acids (in water) before adding M9 salts, magnesium and calcium salts. 

'''M9-CAglu '''contains both: In the basic recipe take 700 ml sterile water and supplement with 100 ml 20% glucose and 100 ml casamino acids before adding M9 salts, magnesium and calcium salts. <br> 

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