Editing Phage Purification (section)

== Material & Reagents ==

The following materials and reagents are needed for the procedure:<br/>A bench microcentrifuge, sterile DNase-free microtubes, aerosol-barrier tips, and a bucket of ice.

TBS (1x) or Tris Buffer Saline: 50 mM Tris-HCl pH 7.5, 150 mM NaCl, sterile, made 1x from commercial 10x stock solution(prevent possible protease contaminations).

PEG/NaCl (5x) stock solution: PEG-8000 20%, NaCl 2.5 M. Dissolve 100 g PEG-8000 (20% w/v) and 75 g NaCl (2.5 M) in 400 ml ddH20 and bring to a final volume of 500 ml by stirring at RT. The solution can be autoclaved (optional) but mixing during the cooling period is required to prevent a phase separation. Store at RT.