Editing Synthesis of TATs-PEG-PEI (section)

== MAL-PEG-SCM, MAL-dPEG 24-NHS ester ==

[[File:MAL-PEG-SCM.gif|RTENOTITLE]]





ThermoFisher SM(PEG)2:

• Reactive groups: NHS ester and maleimide

• Reactive toward: amino and sulfhydryl groups

• Irreversibly crosslink proteins or peptides by flexible PEG spacer arms

• Polyethylene glycol spacer arms help maintain conjugate solubility

• Pure compound with defined structure and molecular weight, ensuring reproducible protein-modification effects

• PEG spacer provides unique advantages, including increased stability, reduced tendency toward aggregation and reduced immunogenicity

• Ideal for performing controlled, two-step protein conjugations (see Sulfo-SMCC)

SM(PEG)n NHS- and maleimide-activated PEG compounds for crosslinking between primary amines (NH2) and sulfhydryl (SH) groups in proteins and other molecules. A complete set of compounds are offered, each having the same heterobifunctional structure (NHS-PEGn-Maleimide) but differing in the number of discrete ethylene glycol units ( n = 2, 4, 6, 8, 12 or 24). The N-hydroxysuccinimide ester (NHS) group reacts specifically and efficiently with lysine and N-terminal amino groups at pH 7-9 to form stable amide bonds. The maleimide group reacts with reduced sulfhydryls at pH 6.5-7.5 to form stable thioether bonds.