Editing Magnetic beads Preparation Methods (section)

=== Reagents ===

(3-Glycidyloxypropyl)trimethoxysilane:&nbsp;

[https://www.tcichemicals.com/eshop/en/us/commodity/G0210/ https://www.tcichemicals.com/eshop/en/us/commodity/G0210/]

(3-acryloxypropyl) trimethoxysilane,&nbsp;100g $38

Sigma&nbsp;[https://www.sigmaaldrich.com/catalog/product/aldrich/440167 https://www.sigmaaldrich.com/catalog/product/aldrich/440167]&nbsp;&nbsp;100 ML 47.30

(3-Glycidyloxypropyl)trimethoxysilane (GPTMS) is a bifunctional organosilane with three methoxy groups on one side and an epoxy ring on the other. The methoxy groups bind well with glass substrates creating a 3D matrix. The epoxy group is reactive with amides, alcohols, thiols and acids. GPTMS is highly reactive in water and can be used as a linking agent between the surface of the silica and the polymeric matrix.



&nbsp;IDA,&nbsp;[https://www.tcichemicals.com/eshop/en/us/commodity/I0006/ https://www.tcichemicals.com/eshop/en/us/commodity/I0006/]&nbsp;I0006&nbsp; &nbsp;Iminodiacetic Acid,&nbsp;500g $41



(5S)-N-(5-Amino-1-carboxypentyl)iminodiacetic Acid, 113231-05-3,&nbsp;for making NTA





[https://www.chemsrc.com/SupplierProduct/113231-05-3_453224.html https://www.chemsrc.com/SupplierProduct/113231-05-3_453224.html]

[https://www.trc-canada.com/product-detail/?CatNum=A603250 https://www.trc-canada.com/product-detail/?CatNum=A603250], 10 g

[http://www.9dingchem.com/113231-05-3.html http://www.9dingchem.com/113231-05-3.html]





[[File:IDA magnet.png|500px|IDA magnet.png]]

Preparation of IDA-modified Fe3O4@SiO2 magnetic microspheres<br/>Dissolve&nbsp; &nbsp;2.5 g IDA in 50mL of a 2mol/L solution of Na2CO3, adjust pH&nbsp; &nbsp;to 10.0 with 10mol/L NaOH.&nbsp;

Stir the IDA solution for several minutes in an ice bath&nbsp;<br/>Add&nbsp;1.6mL&nbsp;of 3-glycidoxypropyltrimethoxysilane&nbsp; &nbsp;into the solution slowly.&nbsp;<br/>After 30 min of stirring at 0°C,&nbsp;<br/>Warm up to 65°C while&nbsp;&nbsp;stirring.&nbsp;<br/>After 6 h, cool the reaction solution&nbsp; to room temperature

Adjust pH to 6.0 with concentrated HCl.&nbsp;

Add 1g of Fe3O4@SiO2 magnetic microspheres&nbsp; &nbsp;to the above solution&nbsp;

Sonicat for 10min.&nbsp;

Heat the suspension&nbsp; at 95°C for 2 h with stirring. T

Separate beads with&nbsp; a permanent magnet<br/>Wash thoroughly with deionized water six times (50mL each time).<br/>Charge of Fe3O4@SiO2–IDA magnetic microspheres with metal ions:&nbsp;Mix 60mg of Fe3O4@SiO2–IDA magnetic microspheres&nbsp; &nbsp;with 50mL of aqueous solution of CuSO4 or NiSO4 or ZnSO4 (10mg/mL).&nbsp;<br/>Stir the mixture agnetically at 100 rpm for 3 h.