Editing Protein purification using intein (section)

=== Preparation of Chitin Column  ===

The chitin column should be washed with '''10 column volumes of the Column Buffer '''prior to the loading of the crude cell extract. The chitin-binding domain (CBD) present in the intein-tag, allows for the affinity purification of the fusion protein using chitin beads. Generally, a column packed with '''10 ml of chitin beads''' (10 ml bed volume or 20 ml chitin beads slurry) should be used for a '''one liter culture''' (adjust the amount of beads according to expression level).