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== Tag Antibodies == {| border="1" cellspacing="1" cellpadding="1" style="width: 721px;" |- | style="width: 100px;" | Name | style="width: 26px;" | Chain | style="width: 47px;" | Clone ID | style="width: 171px;" | Name | style="width: 137px;" | Vector | style="width: 345px;" | Status |- | style="width: 100px;" | vector | style="width: 26px;" | H | style="width: 47px;" | 3129 <br/> | style="width: 171px;" | pCMV5-HA-VH-mIgG2b<br/> | style="width: 137px;" | 2492 pCMV5-mIgG2b-HC<br/> | style="width: 345px;" | 3129-BamH1-F + 3129-Kpn1-F1<br/> |- | style="width: 100px;" | His | style="width: 26px;" | H+L, sc | style="width: 47px;" | 3002<br/> | style="width: 171px;" | pJet1.2-Anti-His Tag 3D5<br/> | style="width: 137px;" | pJet1.2<br/> | style="width: 345px;" | 3129-Kpn1-F1, sequenced, <span style="color:#FF0000;">good, </span>need to subclone into pET30b<br/> |- | style="width: 100px;" | His | style="width: 26px;" | L | style="width: 47px;" | 3001 <br/> | style="width: 171px;" | pCMV5-His-VL-kappa<br/> | style="width: 137px;" | <br/> | style="width: 345px;" | could not clone, contract out to twist bio |- | style="width: 100px;" | <br/> | style="width: 26px;" | H<br/> | style="width: 47px;" | 3135<br/> | style="width: 171px;" | 3135-3-pMiniT2<br/> | style="width: 137px;" | pMiniT2<br/> | style="width: 345px;" | in pMiniT2 vector now, 3135-3-pMiniT2 sequenced, <span style="color:#FF0000;">good, </span>need to subclone into 3129 pCMV5-HA-VH-mIgG2b using BsrG1-Kpn1 |- | style="width: 100px;" | <br/> | style="width: 26px;" | L | style="width: 47px;" | 3001 <br/> | style="width: 171px;" | pCMV5-His-VL-kappa<br/> | style="width: 137px;" | <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px;">3130 pCMV5-HA-VL-mIgKappa</span><br/> | style="width: 345px;" | gene synthesis from twist bio, <span style="color:#FF0000;">to-do</span> |- | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3135 | style="width: 171px;" | <span style="background-color: rgb(251, 251, 239);">pCMV5-His-VH-IgG2b</span><br/> | style="width: 137px;" | 3129 pCMV5-HA-VH-mIgG2b<br/> | style="width: 345px;" | subclone into 3129 pCMV5-HA-VH-mIgG2b using BsrG1-Kpn1 from 3135-3-pMiniT2 <br/> |- | style="width: 100px;" | Myc<br/> | style="width: 26px;" | L | style="width: 47px;" | 3003 <br/> | style="width: 171px;" | 3003-L-5<br/> | style="width: 137px;" | pJet1.2<br/> | style="width: 345px;" | IL2 signal peptide, BamH1-Kpn1<br/> |- | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3003 | style="width: 171px;" | 3003-H-1<br/> | style="width: 137px;" | pJet1.2<br/> | style="width: 345px;" | need to use 3076-myc-BsrG1-F to fix frame shift |- | style="width: 100px;" | <br/> | style="width: 26px;" | L | style="width: 47px;" | 3075<br/> | style="width: 171px;" | pCMV5-Myc-L-kappa<br/> | style="width: 137px;" | 2493 pCMV5-mIgG-kLC<br/> | style="width: 345px;" | completed, subclone from 3003-L5 using BamH1-Kpn1 |- | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | 3076<br/> | style="width: 171px;" | pCMV5-Myc-H-IgG2b<br/> | style="width: 137px;" | 3129 pCMV5-HA-VH-mIgG2b<br/> | style="width: 345px;" | PCR from 3003-H-1 in pJet1.2, using 3076-myc-BsrG1-F and 3076-myc-Nhe1-Kpn1-R<br/> |- | style="width: 100px;" | HA<br/> | style="width: 26px;" | L | style="width: 47px;" | <br/> | style="width: 171px;" | <br/> | style="width: 137px;" | <br/> | style="width: 345px;" | <br/> |- | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | <br/> | style="width: 171px;" | <br/> | style="width: 137px;" | <br/> | style="width: 345px;" | <br/> |- | style="width: 100px;" | <br/> | style="width: 26px;" | L | style="width: 47px;" | <br/> | style="width: 171px;" | <br/> | style="width: 137px;" | <br/> | style="width: 345px;" | <br/> |- | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | <br/> | style="width: 171px;" | <br/> | style="width: 137px;" | <br/> | style="width: 345px;" | <br/> |- | style="width: 100px;" | Flag<br/> | style="width: 26px;" | L | style="width: 47px;" | <br/> | style="width: 171px;" | <br/> | style="width: 137px;" | <br/> | style="width: 345px;" | <br/> |- | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | <br/> | style="width: 171px;" | <br/> | style="width: 137px;" | <br/> | style="width: 345px;" | <br/> |- | style="width: 100px;" | <br/> | style="width: 26px;" | L | style="width: 47px;" | <br/> | style="width: 171px;" | <br/> | style="width: 137px;" | <br/> | style="width: 345px;" | <br/> |- | style="width: 100px;" | <br/> | style="width: 26px;" | H | style="width: 47px;" | <br/> | style="width: 171px;" | <br/> | style="width: 137px;" | <br/> | style="width: 345px;" | <br/> |}
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