Editing Yijia LI 3 19 2018 (section)

== Date:3/21/2018 ==

'''Miniprep of 3166(from PCR)'''

Restriction enzymes:BsrGI+NdeI

Buffer:Fastdigest

Time:30min

'''Result:#1,#3,#5 have bands'''

[[File:IMG 3638.JPG|RTENOTITLE]]

'''Screening of 3166 miniprep'''

1,3,5: BsrGI+SalI

1'',3'',5'':BsrGI''

Time:15min

Buffer:Fastdigest

'''Result:good'''

[[File:IMG 3643.JPG|RTENOTITLE]]

'''Miniprep of 3165pMiniT2_3165_3131'''

3165pMiniT2:1-5 EcoRI

3165:6-10 BsrGI+NdeI

3131:11-15 BssHII+XhoI

Time:30min

'''Result:3165pMiniT2(1-5) have bands,3131(12-15)have bands.redo 3165 digestion.'''

Date:3/21/2018

[[File:IMG 3637.JPG|RTENOTITLE]]

Date:3/21/2018

'''Redo digestion of 3165 from PCR'''

Restriction enzymes:BamHI+BsrGI

Length:151bp

Time:15min

Buffer:Fastsdigest

'''Result:#2,#4 have good bands'''

[[File:IMG 3639.JPG|RTENOTITLE]]

'''Screening of miniprep_3165'''

2,4: BsrGI+SalI

2'',4'':BsrGI

Time:15Min

'''Result:seems good, Smear may caused by high concentration, around 300 ng/ul.'''

[[File:IMG 3642.JPG|RTENOTITLE]]
[[Category:My ELN]]