Editing 1248 pET8XHis-DeepVent-Exo (section)

=== Colony Screening<br>  ===

[[Image:1248 Screening.JPG]] 

All negative, not succesfully transformed. May be because the vector is so light 

Date:&nbsp;July 12, 2012 

[[Image:1248 Screening PCR.JPG]] 

There are positive inserts in lanes 3, 5, 6 and 8, but it is of the incorrect size. Expected is 600 bp but it looks like it is 2x the size 

Date: July 16, 2012<br> 

[[Image:1248 0716 Screening.JPG]]<br> 

Sequencing came back bad, grow more bacteria from bacteria, redid MiniPrep, digested plasmids with RE Nde1 and Nco1. Expected size ~600 bp, redigest tomorrow 

Date:&nbsp;July 17, 2012 

[[Image:0717 Digestion.JPG]] 

Digested sample 1 from MiniPrep yesterday (see lane 1), no band. Maybe digest sample 5