Editing Antibody Design by AI (section)

== Next Generation Sequencing (NGS) sample preparation ==

After each round of phage selection, polyclonal plasmid DNA was prepared using QIAprep Spin Miniprep Kit (Qiagen). In a first PCR the '''CDR-H3 '''was amplified over '''9''' cycles with overhanging primers allowing the annealing of the standard '''TruSeq '''primers in a consecutive step. In the second PCR primers with the TruSeq universal '''forward adapter''' and '''one of the 24 TrueSeq index reverse primer''' were used over '''12''' cycles. The resulting fragment was purified using a 1.5% agarose gel and the Wizard SV Gel and PCR Clean-up System (Promega). DNA concentration was measured using the Qubit DNA High sensitivity kit (Invitrogen). Samples were analyzed on a MiSeq using MiSeq Reagent Kit v3 (Illumina) with '''150 '''forward cycles or on a HiSeq using HiSeq PE Cluster Kit v4 cBot and HiSeq SBS Kit v4 (Illumina) with '''76''' forward cycles.